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Series GSE33882 Query DataSets for GSE33882
Status Public on Aug 25, 2012
Title Musashi2 is required for the self-renewal and pluripotency of embryonic stem cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Recent studies have shown that the RNA binding protein Musashi 2 (Msi2) plays prominent roles during development and leukemia. Additionally, in embryonic stem cells (ESC) undergoing the early stages of differentiation, Msi2 has been shown to associate with Sox2, which is required for the self-renewal of ESC. These findings led us to examine the effects of Msi2 on the behavior of ESC. Using an shRNA sequence that targets Msi2 and a scrambled shRNA sequence, we determined that knockdown of Msi2 disrupts the self-renewal of ESC and promotes their differentiation. Collectively, our findings argue that Msi2 is required to support the self-renewal and pluripotency of ESC.
We used microarrays to better understand global changes in ESC gene expression following the knockdown of the RNA-binding protein Msi2 as compared to control ESC expressing a scrambled shRNA.
 
Overall design Mouse embryonic stem cells (D3) were treated with lentivirus engineered for the expression of a Msi2 targeting shRNA sequence or scrambled (control) shRNA sequence. Following selection for infected cells with puromycin, cells were subcultured at low density and allowed to grow for 4 days (see treatment protocol) before RNA was extracted. RNA was collected and analyzed one time for each of the two samples.
 
Contributor(s) Wuebben EL, Mallanna SK, Cox JL, Rizzino A
Citation(s) 22496868
Submission date Nov 22, 2011
Last update date Mar 04, 2019
Contact name Angie Rizzinio
E-mail(s) arizzino@unmc.edu
Organization name University of Nebraska Medical Center
Department Eppley Institute for Research in Cancer and Allied Diseases
Street address 986805 Nebraska Medical Center
City Omaha
State/province NE
ZIP/Postal code 68198-6805
Country USA
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (2)
GSM838334 ESC Msi2 shRNA
GSM838335 ESC scr shRNA
Relations
BioProject PRJNA148269

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33882_RAW.tar 8.4 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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