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| Status |
Public on Feb 24, 2012 |
| Title |
Mediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Mediator complex is an integrative hub for transcriptional regulation. Here we show that Mediator regulates alternative mRNA processing via its Med23 subunit. Combining tandem affinity purification and mass spectrometry, we identified a number of mRNA processing factors that bind to a soluble recombinant Mediator subunit MED23 but not to several other Mediator components. One of these factors, hnRNP L, specifically interacts with MED23 in vitro and in vivo. Consistently, Mediator partially colocalizes with hnRNP L and the splicing machinery in the cell. Functionally Med23 regulates a subset of hnRNP L-targeted alternative splicing (AS) and alternative cleavage and polyadenylation (APA) events as shown by minigene reporters and exon array analysis. ChIP-seq analysis revealed that Med23 can regulate hnRNP L occupancy at their co-regulated genes. Taken together, these results demonstrate a crosstalk between Mediator and the splicing machinery, suggesting a novel mechanism for coupling mRNA processing to transcription.
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| Overall design |
Examination of hnRNP L and H3K36me3 enrichment in sictrl and si23 Hela cells
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| Contributor(s) |
Huang Y, Yao X, Wang G |
| Citation(s) |
22264826 |
| Submission date |
Nov 22, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
yan huang |
| E-mail(s) |
yhuang02@sibs.ac.cn
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| Phone |
8621-54921085
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| Organization name |
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
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| Lab |
Gang Wang's Lab
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| Street address |
Yueyang Road 320
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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| Samples (4)
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| Relations |
| SRA |
SRP009427 |
| BioProject |
PRJNA148321 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33887_RAW.tar |
1.5 Gb |
(http)(custom) |
TAR (of WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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