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Series GSE33930 Query DataSets for GSE33930
Status Public on Dec 01, 2013
Title Genome-wide mapping of histone methylation in human melanocytes and melanoma cell lines
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Aberrant DNA methylation and histone modifications both contribute to carcinogenesis, but how these two epigenetic factors interact to impact gene expression remain unclear. To address this issue, we studied gene expression profiles, DNA methylation and two key histone modifications (H3K4me3 and H3K27me3), in two types of normal melanocytes (HEMn and HEMa) and two melanoma cell lines SK-MEL-28 and LOXIMVI. Using these data, we analyzed the relationship between epigenetic factors and gene expression status in both normal and melanoma cells, and the impact of epigenetic switches on gene expression during melanomagenesis.
 
Overall design ChIP-seq analysis of H3K4me3 and H3K27me3 in two types of normal melanocytes (HEMn and HEMa) and two melanoma cell lines (SK-MEL-28 and LOXIMVI).
 
Contributor(s) Long H, Lu Y, Issa JJ
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Submission date Nov 23, 2011
Last update date May 15, 2019
Contact name HAI LONG
E-mail(s) longhai021123@gmail.com
Phone 713-834-4296
Organization name University of Texas MD Anderson Cancer Center
Department Leukemia
Lab Issa
Street address 1515 Holcombe Blvd., Unit 425
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (12)
GSM838927 HEMn_H3K4me3
GSM838928 HEMn_H3K27me3
GSM838929 HEMn_H3
Relations
SRA SRP009470
BioProject PRJNA148233

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33930_RAW.tar 1.7 Gb (http)(custom) TAR (of BED, TXT)
GSE33930_README_Processed_data_files.txt 924 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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