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Series GSE34018 Query DataSets for GSE34018
Status Public on Mar 29, 2012
Title Integral roles for Rev-erb alpha and Rev-erb beta in the circadian clock function [Expression array]
Organism Mus musculus
Experiment type Expression profiling by array
Summary The circadian clock acts at the genomic level to coordinate internal behavioral and physiologic rhythms via the CLOCK-BMAL transcriptional heterodimer. Although the nuclear receptors REV-ERBα and β have been proposed to contribute to clock function, their precise roles and importance remain unresolved. To establish their regulatory potential we generated comparative cistromes of both Rev-erb isoforms, which revealed shared recognition at over ~50% of their total sites and extensive overlap with the master clock regulator Bmal. While Rev-erbα has been shown to directly regulate Bmal expression, the cistromic analysis reveals a more profound connection between Bmal and Rev-erbα and β regulatory circuits than previously suspected. Genes within the intersection of the Bmal and Rev-erb cistromes are highly enriched for both clock and metabolic functions. As predicted by the cistromic analysis, dual depletion of Rev-erbα/β function by creating double-knockout mice (DKOs) profoundly disrupted circadian expression of core clock and lipid homeostatic genes. As a result, DKOs show strikingly altered circadian wheel-running behavior and deregulated lipid metabolism. These data reveal an integral role of Rev-erbα/β in clock function as well as provide a cistromic basis for the integration of circadian rhythm and metabolism.
 
Overall design Total RNA was obtained from livers of wild-type and Liver-specific Reverb alpha/beta double knockout mice at ZT 0, 4, 8, 12, 16, and 20.
 
Contributor(s) Yu RT, Cho H, Evans RM
Citation(s) 22460952
Submission date Nov 29, 2011
Last update date Jun 14, 2018
Contact name Ruth T Yu
Organization name Salk Institute
Department Gene Expression Lab
Lab Ronald Evans
Street address 10010 N Torrey Pines Rd
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (24)
GSM840504 Reverb alpha/beta DKO liver_ZT0_rep 1
GSM840505 Reverb alpha/beta DKO liver_ZT0_rep 2
GSM840506 Reverb alpha/beta DKO liver_ZT4_rep 1
This SubSeries is part of SuperSeries:
GSE34020 Integral roles for Rev-erb alpha and Rev-erb beta in the circadian clock function
Relations
BioProject PRJNA156457

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34018_RAW.tar 3.1 Mb (http)(custom) TAR
GSE34018_non_normalized.txt.gz 7.1 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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