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Series GSE34019 Query DataSets for GSE34019
Status Public on Mar 29, 2012
Title Integral roles for Rev-erb alpha and Rev-erb beta in the circadian clock function [ChIP_seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The circadian clock acts at the genomic level to coordinate internal behavioral and physiologic rhythms via the CLOCK-BMAL transcriptional heterodimer. Although the nuclear receptors REV-ERBα and β have been proposed to contribute to clock function, their precise roles and importance remain unresolved. To establish their regulatory potential we generated comparative cistromes of both Rev-erb isoforms, which revealed shared recognition at over ~50% of their total sites and extensive overlap with the master clock regulator Bmal. While Rev-erbα has been shown to directly regulate Bmal expression, the cistromic analysis reveals a more profound connection between Bmal and Rev-erbα and β regulatory circuits than previously suspected. Genes within the intersection of the Bmal and Rev-erb cistromes are highly enriched for both clock and metabolic functions. As predicted by the cistromic analysis, dual depletion of Rev-erbα/β function by creating double-knockout mice (DKOs) profoundly disrupted circadian expression of core clock and lipid homeostatic genes. As a result, DKOs show strikingly altered circadian wheel-running behavior and deregulated lipid metabolism. These data reveal an integral role of Rev-erbα/β in clock function as well as provide a cistromic basis for the integration of circadian rhythm and metabolism.
Overall design Identification of Reverb alpha and Reverb beta binding sites in mouse liver at ZT8
Contributor(s) Yu RT, Cho H, Evans RM
Citation(s) 22460952
Submission date Nov 29, 2011
Last update date May 15, 2019
Contact name Ruth T Yu
Organization name Salk Institute
Department Gene Expression Lab
Lab Ronald Evans
Street address 10010 N Torrey Pines Rd
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (3)
GSM840528 REV-ERB alpha
GSM840529 REV-ERB beta
GSM840530 liver input
This SubSeries is part of SuperSeries:
GSE34020 Integral roles for Rev-erb alpha and Rev-erb beta in the circadian clock function
SRA SRP009472
BioProject PRJNA156459

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Supplementary file Size Download File type/resource
GSE34019_RAW.tar 106.3 Mb (http)(custom) TAR (of BEDGRAPH, TXT)
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Processed data provided as supplementary file
Raw data are available in SRA

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