|
| Status |
Public on Dec 29, 2012 |
| Title |
Dissection of human embryonic stem cells-derived endoderm progenitors by differential cell capture on antibody arrays |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Heterogeneity, shortage of material, and lack of progenitor-specific cell surface markers are major obstacles to elucidating the mechanisms underlying developmental processes. Here we report a proteomic platform that alleviates these difficulties and demonstrate its effectiveness in fractionating heterogeneous cultures of early endoderm derived from human embryonic stem cells. The approach, designated cell-capture antibody array, is based on highly parallel, comparative screening of live cell populations using hundreds of antibodies directed against cell-surface antigens. The results demonstrate the potential of the cell-capture antibody array as a powerful tool for detailed dissection of heterogeneous cellular systems.
|
| |
|
| Overall design |
Genome-wide comparion of mRNA profile in hES-derived CD61+ cells versus CXCR4+ and CXCR4+/CD61- cells using Affymetrix arrays.
|
| |
|
| Contributor(s) |
Sharivkin R, Walker MD, Soen Y |
| Citation(s) |
22580589 |
| Submission date |
Dec 01, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Yoav Soen |
| E-mail(s) |
yoavs@weizmann.ac.il
|
| Organization name |
Weizmann Institute of Science
|
| Street address |
Department of Biological Chemistry, Weizmann Institute of Science
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (5)
|
| GSM841472 |
hESCs-derived CD61+, biological rep1 |
| GSM841473 |
hESCs-derived CXCR4+, biological rep1 |
| GSM841474 |
hESCs-derived CD61+, biological rep2 |
| GSM841475 |
hESCs-derived CXCR4+, biological rep2 |
| GSM841476 |
hESCs-derived CXCR4+/CD61-, biological rep2 |
|
| Relations |
| BioProject |
PRJNA150001 |
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