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| Status |
Public on Jan 07, 2013 |
| Title |
Aberrant microRNA expression in radiation-induced rat mammary cancer: the potential role of miR-194 overexpression in cancer cell proliferation. |
| Organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Aberrant expression of microRNAs (miRNAs) is frequently associated with a variety of cancers, including breast cancer. We and others have demonstrated that radiation-induced rat mammary cancer exhibits a characteristic gene expression profile and a random increase in aberrant DNA copy number; however, the role of aberrant miRNA expression is unclear. We performed a microarray analysis of frozen samples of eight mammary cancers induced by gamma-irradiation (2 Gy), eight spontaneous mammary cancers, and seven normal mammary samples. We found that a small set of miRNAs was characteristically overexpressed in radiation-induced cancer. Quantitative RT-PCR analysis confirmed that miR-135b, miR-192, miR-194, and miR-211 were significantly upregulated in radiation-induced mammary cancer compared with spontaneous cancer and normal mammary tissue. The expression of miR-192 and miR-194 also was upregulated in human breast cancer cell lines compared with non-cancer cells. Manipulation of the miR-194 expression level using a synthetic inhibiting RNA produced a small but significant suppression of cell proliferation and upregulation in the expression of several genes that are suggested to act as tumor suppressors in MCF-7 and T47D breast cancer cells. Thus, the induction of rat mammary cancer by radiation involves aberrant expression of miRNAs, which may favor cell proliferation.
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| Overall design |
We performed miRNA microarray analysis on mammary carcinomas in Sprague-Dawley rat to identify radiation-specific miRNA expression patterns compared with spontaneous mammary carcinomas and normal mammary tissues.
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| Contributor(s) |
Iizuka D, Imaoka T, Nishimura M, Kawai H, Suzuki F, Shimada Y |
| Citation(s) |
23273170 |
| Submission date |
Dec 02, 2011 |
| Last update date |
Jan 09, 2013 |
| Contact name |
Daisuke IIZUKA |
| Organization name |
Research Institutes for Quantum Science and Technology
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| Street address |
4-9-1, Anagawa, Inage-ku
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| City |
Chiba |
| ZIP/Postal code |
263-8555 |
| Country |
Japan |
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| Platforms (1) |
| GPL10344 |
Agilent-019159 Rat miRNA Microarray (miRBase release 9.2 miRNA ID version) |
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| Samples (23)
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| GSM842107 |
Spontaneous mammary carcinoma, K0179-1 |
| GSM842108 |
Spontaneous mammary carcinoma, K0265-3 |
| GSM842109 |
Spontaneous mammary carcinoma, K0672-2 |
| GSM842110 |
Spontaneous mammary carcinoma, K0677-1 |
| GSM842111 |
Spontaneous mammary carcinoma, K0679-2 |
| GSM842112 |
Spontaneous mammary carcinoma, M1596-1 |
| GSM842113 |
Spontaneous mammary carcinoma, M1605-2 |
| GSM842114 |
Spontaneous mammary carcinoma, M1695-1 |
| GSM842115 |
Radiation-induced mammary carcinoma, M1177-1 |
| GSM842116 |
Radiation-induced mammary carcinoma, M1233-1 |
| GSM842117 |
Radiation-induced mammary carcinoma, M1413-1 |
| GSM842118 |
Radiation-induced mammary carcinoma, M1414-1 |
| GSM842119 |
Radiation-induced mammary carcinoma, M1415-3 |
| GSM842120 |
Radiation-induced mammary carcinoma, M1466-2 |
| GSM842121 |
Radiation-induced mammary carcinoma, M1467-1 |
| GSM842122 |
Radiation-induced mammary carcinoma, M1612-3 |
| GSM842123 |
Normal mammary tissue, Normal1 |
| GSM842124 |
Normal mammary tissue, Normal2 |
| GSM842125 |
Normal mammary tissue, Normal3 |
| GSM842126 |
Normal mammary tissue, Normal4 |
| GSM842127 |
Normal mammary tissue, Normal5 |
| GSM842128 |
Normal mammary tissue, Normal6 |
| GSM842129 |
Normal mammary tissue, Normal7 |
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| Relations |
| BioProject |
PRJNA149871 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34119_RAW.tar |
9.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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