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Series GSE34170 Query DataSets for GSE34170
Status Public on Nov 22, 2012
Title Dual knockdown of Esrrb and Sox2 in mouse embryonic stem cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Esrrb, Sox2 or Esrrb and Sox2 were knocked down in mouse ES cells using shRNA plasmid pSUPER.puro containing shRNAs from the publications Feng et al., 2009 and Rodda et al., 2005.
Knockdown was transfected into mouse ES cells using lipofectamine and then cells were selected for knockdown using puromycin.
RNA was harvested after 2 days of knockdown.
 
Overall design Esrrb and Sox2 were knocked down in mouse ES cells either individually or together, cells were selected with puromycin and RNA harvested and subjected to microarray after 2 days.
 
Contributor(s) Robson P, Hutchins AP
Citation(s) 23169531
Submission date Dec 05, 2011
Last update date Jun 14, 2018
Contact name Andrew P Hutchins
E-mail(s) andrewh@sustech.edu.cn
Organization name Southern University of Science and Technology
Department Bioinformatics
Lab Bioinformatics and Genomics
Street address 1088 Xueyuan Rd
City Shenzhen
ZIP/Postal code 518055
Country China
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (4)
GSM843384 ES cells GFP shRNA rep1-rep4
GSM843385 ES cells Esrrb shRNA rep1-rep4
GSM843386 ES cells Sox2 shRNA rep1-rep4
Relations
BioProject PRJNA149721

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34170_RAW.tar 3.1 Mb (http)(custom) TAR
GSE34170_non-normalized.txt.gz 1.6 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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