|
Status |
Public on Dec 08, 2011 |
Title |
C2C12 murine myoblast cell line and 48 h 10 uM BIO treated C2C12 cellulate |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
In urodele amphibians, limb regeneration involves the dedifferentiation of muscle myotubes into single cells that may acquire pluripotent potential. We have employed small molecules (myoseverin and BIO) to attempt to reproduce this behavior in mammalian muscle culture. C2C12 myotubes derived from the C2C12 myoblast cell line were induced to undergo cellularization by myoseverin treatment, which destabilizes tubulin filaments. The GSK-3 inhibitor, BIO, was then used to induce dedifferentiation. Induce neuron formation; the cells were incubated with 250 nM reversine for 48 h, and neural induction media (DMEM/F12 supplemented with N2 (Invitrogen)) and 1.5 uM all-trans retinoic acid for 7 days. C2C12 murine myoblast cell line and 48 h 10 uM BIO treated C2C12 cellulate (derived by 20 M myoseverin treatment for 48 h)
|
|
|
Overall design |
C2C12 myoblasts were differentiated into myotubes with 2%horse serum in DMEM for 8 days (from 2-4 d, 10 uM AraC treatment was also used to kill any remaining myoblasts). Next, myotubes were cellularized by 20 uM myoseverin treatment for 48 h. 24 h after myoseverin treatment, myotubes were treated with 10 uM BIO for 2d.
|
|
|
Contributor(s) |
Williams DR, Kim W |
Citation(s) |
22270490 |
Submission date |
Dec 07, 2011 |
Last update date |
Jan 12, 2017 |
Contact name |
Darren Reece Williams |
E-mail(s) |
darren@gist.ac.kr
|
Phone |
8210-8780-0915
|
Organization name |
Gwangju Institute of Science and Technology
|
Department |
School of Life Sciences
|
Lab |
New Drug Targets Laboratory
|
Street address |
1 Oryong-dong
|
City |
Gwangju |
ZIP/Postal code |
500-712 |
Country |
South Korea |
|
|
Platforms (1) |
GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
|
Samples (3) |
GSM845336 |
Ex Vivo C2C12 myoblast |
GSM845337 |
Ex Vivo Cellulate 2 days 2.5 uM BIO |
GSM845338 |
Ex Vivo Cellulate 2 days 2.5 uM BIO 7 days neurogenic media |
|
Relations |
BioProject |
PRJNA149805 |