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Series GSE34244 Query DataSets for GSE34244
Status Public on Dec 08, 2011
Title REST Couples Loss of Pluripotency with Neural Induction and Neural Differentiation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Neural differentiation of embryonic stem cells (ESCs) requires coordinated repression of the pluripotency regulatory programme and reciprocal activation of the neurogenic regulatory programme. Upon neural induction, ESCs rapidly repress expression of pluripotency genes followed by staged activation of neural progenitor and differentiated neuronal and glial genes. The transcriptional factors that underlie pluripotency are partially characterized, whereas those underlying neural induction are much less explored, and the factors that coordinate these two developmental programs are completely unknown. One transcription factor, REST (RE1 silencing transcription factor), has been linked with terminal differentiation of neural progenitors, and more recently and controversially, with control of pluripotency. Here, we show that in the absence of REST, coordination of pluripotency and neural induction is lost and there is a resultant delay in repression of pluripotency genes and a precocious activation of both neural progenitor and differentiated neuronal and glial genes. Further, we show that REST is not required for production of radial glia-like progenitors but is required for their subsequent maintenance and differentiation into neurons, oligodendrocytes and astrocytes. We propose that REST acts as a regulatory hub that coordinates timely repression of pluripotency with neural induction and neural differentiation.
 
Overall design We have investigated the role of REST in the coupling of pluripotency and neural induction and neurogenesis using Rest-null mouse ESCs (mESCs) in conjunction with a novel mESC neural differentiation protocol. Differential expression between Rest-null ESCs and controls was tested using Illumina Mouse Ref-8 v2 BeadArray technology. Three replicates were used for Rest-null and three for control. Data analysis was performed using Bioconductor libraries beadarray and limma.
 
Contributor(s) Soldati C, Bithell A, Johnston C, Wong KY, Teng SW, Beglopoulos V, Stanton LW, Buckley NJ
Citation(s) 22162260
Submission date Dec 07, 2011
Last update date Jun 14, 2018
Contact name Caroline Emma Johnston
E-mail(s) caroline.johnston@iop.kcl.ac.uk
Organization name Kings College London
Street address 125 Coldharbour Lane
City London
ZIP/Postal code SE5 9NU
Country United Kingdom
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM845680 FR1
GSM845681 FR2
GSM845682 FR3
Relations
BioProject PRJNA149763

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34244_RAW.tar 3.1 Mb (http)(custom) TAR
GSE34244_complete_raw_data.txt.gz 1.7 Mb (ftp)(http) TXT
GSE34244_limma_results_RestKO_vs_Restflox.txt.gz 1.9 Mb (ftp)(http) TXT
GSE34244_non-normalized.txt.gz 721.7 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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