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| Status |
Public on Jul 18, 2013 |
| Title |
Dietary heme modulates microbiota and mucosa of mouse colon without significant host-microbe cross talk |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Previously, we showed that dietary heme injured the colonic surface epithelium and induced hyperproliferation by changing the surface to crypt signaling. In this study we investigated whether bacteria play a role in this changed signaling. Dietary heme increased the Bacteroidetes and decreased the Firmicutes in colonic content. This shift was caused by a selective susceptibility of Gram-positive bacteria to the heme cytotoxic fecal waters, which is not observed for Gram-negative bacteria allowing expansion of the Gram-negative community. The increased amount of Gram-negative bacteria increased LPS exposure to colonocytes, however, there is no appreciable immune response detected in the heme-fed mice. There were no signs of sensing of the bacteria by the mucosa, as changes in TLR signaling were not present. This lack of microbe-host cross talk indicated that the changes in microbiota do not play a causal role in the heme-induced hyperproliferation.
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| Overall design |
Mice received control or heme diet for 14 days, whereafter pooled colon samples were analysed on microarrays.
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| Contributor(s) |
IJssennagger N, Rijnierse A, Muller M, Meer Rv |
| Citation(s) |
23239972 |
| Submission date |
Dec 08, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
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| Organization name |
Wageningen University
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| Department |
Div. Human Nutrition & Health
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| Lab |
Nutrition, Metabolism & Genomics Group
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| Street address |
HELIX, Stippeneng 4
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| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA149753 |
