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| Status |
Public on Dec 13, 2011 |
| Title |
dKDM2 couples histone H2A ubiquitylation to histone H3 demethylation during Polycomb group silencing. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Transcription regulation involves enzyme-mediated changes in chromatin structure. Here, we describe a novel mode of histone crosstalk during gene silencing, in which histone H2A monoubiquitylation is coupled to the removal of histone H3 Lys 36 dimethylation (H3K36me2). This pathway was uncovered through the identification of dRING-associated factors (dRAF), a novel Polycomb group (PcG) silencing complex harboring the histone H2A ubiquitin ligase dRING, PSC and the F-box protein, and demethylase dKDM2. In vivo, dKDM2 shares many transcriptional targets with Polycomb and counteracts the histone methyltransferases TRX and ASH1. Importantly, cellular depletion and in vitro reconstitution assays revealed that dKDM2 not only mediates H3K36me2 demethylation but is also required for efficient H2A ubiquitylation by dRING/PSC. Thus, dRAF removes an active mark from histone H3 and adds a repressive one to H2A. These findings reveal coordinate trans-histone regulation by a PcG complex to mediate gene repression.
Microarray analysis of dKDM2, dRING, PC, PH and PSC target genes in Drosophila S2 cells
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| Overall design |
Expression profiles of Drosophila S2 cells RNAi depleted (KD) for dKDM2, dRING, PC, PH or PSC were compared with expression profile of untreated S2 cells (Mock)
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| Contributor(s) |
Lagarou A, Mohd-Sarip A, Moshkin YM, Verrijzer CP |
| Citation(s) |
18923078 |
| Submission date |
Dec 12, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yuri Moshkin |
| E-mail(s) |
i.mochkine@erasmusmc.nl
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| Phone |
0031-10-7043335
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| Organization name |
Erasmus University Medical Center
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| Street address |
Dr. Molewaterplein 50
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| City |
Rotterdam |
| ZIP/Postal code |
3015 GE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (29)
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| GSM848153 |
S2 cells, biological rep4 |
| GSM848154 |
S2 cells, biological rep5 |
| GSM848155 |
S2 cells, biological rep6 |
| GSM848156 |
S2 cells, biological rep7 |
| GSM848157 |
S2 cells, biological rep8 |
| GSM848158 |
S2 cells, biological rep9 |
| GSM848159 |
S2 cells, biological rep10 |
| GSM848160 |
S2 cells, biological rep11 |
| GSM848161 |
S2 cells, biological rep12 |
| GSM848162 |
S2 cells, biological rep13 |
| GSM848163 |
S2 cells, biological rep14 |
| GSM848164 |
S2 cells dKDM2 RNAi, biological rep1 |
| GSM848165 |
S2 cells dKDM2 RNAi, biological rep2 |
| GSM848166 |
S2 cells dKDM2 RNAi, biological rep3 |
| GSM848167 |
S2 cells dRING RNAi, biological rep1 |
| GSM848168 |
S2 cells dRING RNAi, biological rep2 |
| GSM848169 |
S2 cells dRING RNAi, biological rep3 |
| GSM848170 |
S2 cells PC RNAi, biological rep1 |
| GSM848171 |
S2 cells PC RNAi, biological rep2 |
| GSM848172 |
S2 cells PC RNAi, biological rep3 |
| GSM848173 |
S2 cells PH RNAi, biological rep1 |
| GSM848174 |
S2 cells PH RNAi, biological rep2 |
| GSM848175 |
S2 cells PH RNAi, biological rep3 |
| GSM848176 |
S2 cells PSC RNAi, biological rep1 |
| GSM848177 |
S2 cells PSC RNAi, biological rep2 |
| GSM848178 |
S2 cells PSC RNAi, biological rep3 |
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| Relations |
| BioProject |
PRJNA149565 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34390_RAW.tar |
55.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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