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Series GSE34442 Query DataSets for GSE34442
Status Public on Aug 30, 2013
Title The Autonomous Hepatocyte Clock Controls Acetaminophen Bioactivation and Chronotoxicity [Liver]
Organism Mus musculus
Experiment type Expression profiling by array
Summary The diurnal variation in acetaminophen (APAP) hepatotoxicity (“chronotoxicity”) is thought to be due to oscillations in xenobiotic metabolism that are influenced by the circadian phases of feeding or fasting. Because of APAP’s relevance to human poisoning, we set out to determine the relative contributions of the central clock in the SCN and the autonomous clock in the hepatocyte in modulating the chronotoxicity of APAP. Using a conditional null allele of Mop3 (ArntL, Bmal1) we are able to delete the clock from hepatocytes while keeping the central and other peripheral clocks intact (eg, those controlling food intake). Our data from this hepatocyte-null mouse model suggests that, while the central circadian clock modulates some detoxification pathways indirectly by driving activity patterns and feeding rhythms, the autonomous hepatocyte circadian clock controls major aspects of APAP bioactivation independent of feeding rhythms.
 
Overall design 10-20 week old Mop3fxfx mice positive or negative for Cre-recombinase driven by the albumin promoter, housed in 12 hour light:12 dark, ad lib feeding and drinking conditions were sacrificed every four hours over two separte days beginning at ZT0. A two color, reference design experiment in which liver RNA from at least 3 mice per timepoint were pooled and labeled with Cy3 and hybridized according to Agilent protocols against a reference pool of RNA madeup from respective tissue taken from 10 week Mop3fxfx and Mop3fxfxCreAlb mice which was labeled with Cy5.
 
Contributor(s) Johnson BP, Bradfield CA
Citation(s) 25512522
Submission date Dec 14, 2011
Last update date May 10, 2018
Contact name Brian P Johnson
Phone 608-262-1209
Organization name UW-Madison
Department Oncology
Lab Chris Bradfield
Street address 1400 University Ave
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (24)
GSM849228 Mop3fxfx_liver_10week_ZT0
GSM849229 Mop3fxfx_liver_10week_ZT4
GSM849230 Mop3fxfx_liver_10week_ZT8
This SubSeries is part of SuperSeries:
GSE34444 The Autonomous Hepatocyte Clock Controls Acetaminophen Bioactivation and Chronotoxicity
Relations
BioProject PRJNA156377

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34442_RAW.tar 350.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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