|
| Status |
Public on Dec 01, 2012 |
| Title |
Differential Expression of Iron Acquisition Genes by Brucella melitensis and Brucella canis During Macrophage Infection (part 1) |
| Platform organism |
Brucella melitensis |
| Sample organism |
Brucella melitensis bv. 1 str. 16M |
| Experiment type |
Expression profiling by array
|
| Summary |
Brucella melitensis and Brucella canis differ by ~75 genes yet B. melitensis is highly virulent for humans while B. canis is considered rarely pathogenic. No identified bacterial factors or mechanisms account for this difference in virulence. To identify functional differences of these two bacteria, gene transcription was examined during infection of murine macrophages and compared to bacteria grown in broth. Our analysis identified transcriptional differences in macrophage infection between B. melitensis and B. canis genes involved in iron transport. Increased transcription of the TonB, enterobactin, and ferric anguibactin transport systems were observed in B. canis but not B. melitensis during infection of macrophages. Therefore, iron appears as an important requirement during the first 24h of infection by B. canis but not for B. melitensis and provides strategies for controlling these pathogens.
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| Overall design |
Comparison of total bacterial RNA from Brucella melitensis infected murine macrophages to broth grown bacteria
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| Contributor(s) |
Eskra L, Covert J, Glasner J, Splitter G |
| Citation(s) |
22403618 |
| Submission date |
Dec 16, 2011 |
| Last update date |
Mar 04, 2013 |
| Contact name |
Gary Splitter |
| E-mail(s) |
splitter@svm.vetmed.wisc.edu
|
| Phone |
6082621837
|
| Fax |
6082627420
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Pathobiological Sciences
|
| Street address |
1656 Linden Dr
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15023 |
NimbleGen Brucella melitensis 3K A4357-001-01 |
|
| Samples (4)
|
| GSM850205 |
B. melitensis, broth culture rep1 |
| GSM850206 |
B. melitensis, broth culture rep2 |
| GSM850207 |
B. melitensis from infected cells (24h), rep1 |
| GSM850208 |
B. melitensis from infected cells (24h), rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE34504 |
Differential Expression of Iron Acquisition Genes by Brucella melitensis and Brucella canis During Macrophage Infection |
|
| Relations |
| BioProject |
PRJNA156335 |
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