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Status |
Public on Jun 09, 2012 |
Title |
MicroRNA expression data from leukemia and breast cancer cell lines with/without treatment with microparticles |
Platform organism |
synthetic construct |
Sample organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by array
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Summary |
Microparticles (MPs) comprise the major source of systemic RNA including microRNA (miRNA), the aberrant expression of which appears to be associated with stage, progression and spread of many cancers. We have shown MPs to transfer multidrug resistance proteins accross both haematological and and non-haematological cancers. using microarray miRNA profiling analysis we now analyze changes in miRNA profiles of both cancer types following microparticle transfer. We identified certain upregulated miRNAs in both cancer types.
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Overall design |
Total RNA was extracted and pooled from duplicate experiments for hybridization on Affymetrix microarrays from (i) the parental drug sensitive leukaemia (CEM) or breast cancer (MCF-7) cells, (ii) their Multidrug Resistant strains leukaemia (VLB100) or breast cancer ( DX cells), (iii) the microparticles isolated from the resistant cells: VLBMP or DXMP, and (iv) the cocultured samples: sensitive cell co-incubated with MPs from their resistant cells ( leukaemia: CEM+VLBMP) or(breast cancer: MCF-7+DXMP). We sought to examine the miRNA profiles of the drug sensitve cells after MP transfer from drug resistant cells across leukaemia nd breact cancer cell lines.
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Contributor(s) |
Jaiswal R, Gong J, Luk F, Bebawy M |
Citation(s) |
25177548 |
Submission date |
Dec 19, 2011 |
Last update date |
Oct 22, 2019 |
Contact name |
Ritu Jaiswal |
E-mail(s) |
rjai9330@uni.sydney.edu.au
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Organization name |
The University of Sydney
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Street address |
92 - 94 Parramatta Rd
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City |
Sydney |
ZIP/Postal code |
2006 |
Country |
Australia |
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Platforms (1) |
GPL8786 |
[miRNA-1] Affymetrix Multispecies miRNA-1 Array |
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Samples (8)
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GSM851035 |
Breast cancer drug sensitve cell MCF-7 |
GSM851036 |
Breast coculture MCF-7 + DX microparticles |
GSM851037 |
Breast drug resistant cell DX |
GSM851038 |
Breast drug resistant DX microparticles |
GSM851039 |
Leukaemia drug sensitve cell CEM |
GSM851040 |
Leukaemia coculture CEM + VLB100 microparticles |
GSM851041 |
Leukaemia drug resistant cell VLB100 |
GSM851042 |
Leukaemia drug resistant VLB100 microparticles |
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Relations |
BioProject |
PRJNA151529 |