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Series GSE34560 Query DataSets for GSE34560
Status Public on Jun 09, 2012
Title MicroRNA expression data from leukemia and breast cancer cell lines with/without treatment with microparticles
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Microparticles (MPs) comprise the major source of systemic RNA including microRNA (miRNA), the aberrant expression of which appears to be associated with stage, progression and spread of many cancers. We have shown MPs to transfer multidrug resistance proteins accross both haematological and and non-haematological cancers.
using microarray miRNA profiling analysis we now analyze changes in miRNA profiles of both cancer types following microparticle transfer. We identified certain upregulated miRNAs in both cancer types.
 
Overall design Total RNA was extracted and pooled from duplicate experiments for hybridization on Affymetrix microarrays from (i) the parental drug sensitive leukaemia (CEM) or breast cancer (MCF-7) cells, (ii) their Multidrug Resistant strains leukaemia (VLB100) or breast cancer ( DX cells), (iii) the microparticles isolated from the resistant cells: VLBMP or DXMP, and (iv) the cocultured samples: sensitive cell co-incubated with MPs from their resistant cells ( leukaemia: CEM+VLBMP) or(breast cancer: MCF-7+DXMP). We sought to examine the miRNA profiles of the drug sensitve cells after MP transfer from drug resistant cells across leukaemia nd breact cancer cell lines.
 
Contributor(s) Jaiswal R, Gong J, Luk F, Bebawy M
Citation(s) 25177548
Submission date Dec 19, 2011
Last update date Oct 22, 2019
Contact name Ritu Jaiswal
E-mail(s) rjai9330@uni.sydney.edu.au
Organization name The University of Sydney
Street address 92 - 94 Parramatta Rd
City Sydney
ZIP/Postal code 2006
Country Australia
 
Platforms (1)
GPL8786 [miRNA-1] Affymetrix Multispecies miRNA-1 Array
Samples (8)
GSM851035 Breast cancer drug sensitve cell MCF-7
GSM851036 Breast coculture MCF-7 + DX microparticles
GSM851037 Breast drug resistant cell DX
Relations
BioProject PRJNA151529

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Supplementary file Size Download File type/resource
GSE34560_RAW.tar 1.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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