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Series GSE34562 Query DataSets for GSE34562
Status Public on Dec 31, 2012
Title IL-6R identifies early differentiated human effector memory CD8+ T cells that potently expand and produce IL-2 and IL-13
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We found a unique subset of effector memory (EM) CD8+ T cells that expressed high levels of IL-6 receptor in human peripheral blood. These cells which also expressed high levels of IL-7Ra (referred to as IL-6R high IL-7Rahigh cells) had the a distinct gene expression profile and cellular characteristics compared to other EM CD8+ T cells. IL-6R high IL-7Ra high cells were early differentiated EM CD8+ T cells with decreased expression of T-bet, KLRG1, perforin and granzyme B. These cells had increased cell proliferation likely secondary to enhanced IL-2 production and high affinity IL-2R expression. IL-6R high IL-7Ra high EM CD8+ T cells exclusively produced high levels of IL-2, IL-5, IL-9 and IL-13 although IFN-r was produced by this cell subset and other EM CD8+ T cells. Of interest, IL-6R high IL-7Ra high EM CD8+ T cells expanded in the peripheral blood of patients with chronic obstructive pulmonary disease (COPD) and asthma where CD8+ T cells, IL-13 and IFN-r are suggested to be involved in the pathogenesis. Being the early-differentiated EM CD8+ T cells with a potent capacity to proliferate, survive and generate multiple cytokines, IL-6R high IL-7Ra high EM CD8+ T cells may serve as a primary reservoir for effector CD8+ T cells which potently expand and produce cytokines upon immune stimulation.
 
Overall design Duplicate experiments were performed for each condition. In each condition, we independently prepared total RNA using the RNeasy mini kit (Qiagen) and assessed RNA integrity using Bioanalyzer 2100 (Agilent)- RINs were close to 10 for all samples. RNA was then amplified and hybridized to the Illumina HumanHT-12 v4.0 BeadChip, according to Illumina standard protocols.
 
Contributor(s) Kang I, Hwang D
Citation(s) 25390970
Submission date Dec 19, 2011
Last update date Apr 29, 2019
Contact name Sungyong You
E-mail(s) Sungyong.You@cshs.org
Organization name Cedars-Sinai Medical Center
Department Surgery
Lab Freeman Lab.
Street address Beverly Blvd 8400
City LA
State/province CA
ZIP/Postal code 90048
Country USA
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (6)
GSM851091 CD8EM IL6Rlow IL7Rlow 1
GSM851092 CD8EM IL6Rlow IL7Rlow 2
GSM851093 CD8EM IL6Rlow IL7Rhigh 1
Relations
BioProject PRJNA151037

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34562_RAW.tar 26.2 Mb (http)(custom) TAR
GSE34562_non-normalized.txt.gz 1.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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