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Status |
Public on Apr 22, 2013 |
Title |
Genome-wide analysis of monocytes and T cells' response to interferon beta |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
The research hypothesis was that the unique expression profile of monocytes in response to Interferon-beta (IFN-β) might be masked by the response profile of the more abundant T cells. The analysis of monocytes response may highlight novel IFN-β functions and pathways that have not been recognized previously. The aim of this study was to characterized the IFN-β transcriptional response in monocytes, a small but influential cell-subset, using gene expression profile and pathway analysis. The expression profiles of purified human monocytes and T cells pulse with IFN-β overnight were compared, and revealed 276 and 42 Differentially Expressed Genes (DEGs) in monocytes and T cells respectively, with only 5 genes common to both sets. Many of the DEGs detected in monocytes were novel with respect to the recognized IFN-β response, and included genes involved in immune-specific activities, communication between cells, regulation of apoptosis, cell migration, and protein translation. Network and pathway analysis of the T cells response revealed the familiar immune pathways, response to virus pathways, and IFN canonical pathway. In monocytes, however, we detected along with known IFN-β-related pathways, such as cell migration also pathways that had not been previously described in the context of IFN-β response, such as the High-Mobility Group Box-1 (HMGB1) signaling pathway. However, most DEGs in monocytes did not cluster to specific networks or canonical pathways. The monocyte-specific DEGs and pathways described herein are beyond the current knowledge regarding the biology of the IFN-β response and the differential response to IFN-β of monocytes versus T cells described emphasizes the importance of cell-specific studies for elucidating the spectrum of cytokine-mediated immunomodulation.
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Overall design |
Monocytes and T lymphocytes were isolated from 3 healthy volunteers. For each sample the expression profile of untreated cells was compared to the profile of IFN-β treated cells, i.e. 4 samples from each donor (2 cell types, with and without treatment), 12 samples total.
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Contributor(s) |
Henig N, Avidan N, Paperna T, Pinter RY, Miller A |
Citation(s) |
23626809 |
Submission date |
Dec 21, 2011 |
Last update date |
Feb 18, 2019 |
Contact name |
Noa Henig |
Organization name |
Technion Israel Institute of Technology
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Department |
Faculty of Medicine
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Street address |
Efron st.
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City |
Haifa |
ZIP/Postal code |
31096 |
Country |
Israel |
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Platforms (1) |
GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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Samples (12)
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GSM852149 |
T lymphocytes not treated with IFN-β, individual 1 |
GSM852150 |
T lymphocytes treated with IFN-β, individual 1 |
GSM852151 |
Monocytes not treated with IFN-β, individual 1 |
GSM852152 |
Monocytes treated with IFN-β, individual 1 |
GSM852153 |
T lymphocytes not treated with IFN-β, individual 2 |
GSM852154 |
T lymphocytes treated with IFN-β, individual 2 |
GSM852155 |
Monocytes not treated with IFN-β, individual 2 |
GSM852156 |
Monocytes treated with IFN-β, individual 2 |
GSM852157 |
T lymphocytes not treated with IFN-β, individual 3 |
GSM852158 |
T lymphocytes treated with IFN-β, individual 3 |
GSM852159 |
Monocytes not treated with IFN-β, individual 3 |
GSM852160 |
Monocytes treated with IFN-β, individual 3 |
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Relations |
BioProject |
PRJNA151275 |
Supplementary file |
Size |
Download |
File type/resource |
GSE34627_RAW.tar |
6.3 Mb |
(http)(custom) |
TAR |
GSE34627_non-normalized.txt.gz |
5.3 Mb |
(ftp)(http) |
TXT |
Processed data included within Sample table |
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