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| Status |
Public on Dec 31, 2012 |
| Title |
PrlR regulon in Brucella melitensis 16M |
| Organism |
Brucella melitensis bv. 1 str. 16M |
| Experiment type |
Expression profiling by array
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| Summary |
Investigation of whole genome gene expression level changes in a Brucella melitensis delta prlr mutant compared to the wild type strain.
The mutants analyzed in this study are further described in A. Mirabella, R-M Yanez, R.M. Delrue, S. Uzureau, M.S. Zygmunt, A. Cloeckaert, X. De Bolle, J.J. Letesson (2012). The two component system PrlS/PrlR of Brucella melitensis is required for persistence in mice and appears to respond to ionic strength. Microbiology
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| Overall design |
A six chip study using total RNA recovered from three separate wild-type cultures of Brucella melitensis 16M and three separate cultures of a prlR mutant strain. Each chip measures the expression level of 3,198 genes from Brucella melitensis 16M with nineteen 60 mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.
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| Contributor(s) |
Mirabella A, Yanez Villanueva R, Lemaire J, De Bolle X, Letesson J |
| Citation(s) |
22859617 |
| Submission date |
Dec 21, 2011 |
| Last update date |
Apr 03, 2013 |
| Contact name |
LETESSON Jean-Jacques marie |
| Organization name |
University of Namur
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| Street address |
61 rue de Bruxelles
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| City |
NAMUR |
| ZIP/Postal code |
5000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL5730 |
FUNDP/URBM_Brucella.melitensis.16M_v.1.0 |
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| Samples (6)
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| GSM852220 |
Brucella melitensis 16M wild-type OD 600nm 0.3 rep1 |
| GSM852221 |
Brucella melitensis 16M wild-type OD 600nm 0.3 rep2 |
| GSM852223 |
Brucella melitensis 16M wild-type OD 600nm 0.3 rep3 |
| GSM852225 |
Brucella melitensis delta prlR mutant strain OD 600nm 0.3 rep1 |
| GSM852226 |
Brucella melitensis delta prlR mutant strain OD 600nm 0.3 rep2 |
| GSM852228 |
Brucella melitensis delta prlR mutant strain OD 600nm 0.3 rep3 |
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| Relations |
| BioProject |
PRJNA151287 |
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