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Series GSE34645 Query DataSets for GSE34645
Status Public on Mar 27, 2013
Title MiRNA expression profile in murine early atherosclerotic plaque compared with un-diseased arterial tissue
Organism Mus musculus
Experiment type Expression profiling by RT-PCR
Summary Aberrantly expressed miRNAs contribute to developmental abnormalities and diseases such as cancer, diabetes, and cardiovascular disorders, by hybridizing to specific mRNA targets and repressing their translation into proteins. Although miRNA expression signature is characterized in the process of neointimal thickening during proliferative vascular diseases such as atherosclerosis, so far global miRNA expression profiling in the different stages of atherosclerosis is completely unknown. We explored stage-specific microRNA signatures in the progress of atherosclerosis in hyperlipidemia mouse model, which may help to identify the critical miRNAs contributing atherosclerotic development and stabilization.
 
Overall design Female apoe-/- mice (6-8 weeks, Jackson Laboratory) were fed a high fat diet (HFD, 21% crude fat, 0.15% cholesterol and 19.5% casein, Altromin, Germany) for 3 or 10 months (N=3-4). Serial sections (20 µm thick) of aortic roots and carotid arteries from these mice were mounted on membrane mounted metal frame slides (MMI), deparaffinized under RNase-free conditions, and completely dried. Laser capture microdissection (LCM) was performed with a laser microdissection system (MMI cellcut plus, Molecular Machines and Industries, Switzerland) assembled onto an inverted microscope (Olympus IX71). Plaque tissue or morphologically normal vessel wall of at least 40 sections per mouse were collected. RNA was isolated using RecoverAll total nucleic acid isolation kit (Applied Biosystems) according to the manufacturer’s instructions.
 
Contributor(s) Schober A, Jahantigh MN, Wei Y
Citation(s) 23513069
Submission date Dec 22, 2011
Last update date Mar 27, 2013
Contact name Andreas Schober
E-mail(s) aschober@med.lmu.de
Organization name Ludwig-Maximilians-University
Department Institute for Cardiovascular Prevention
Street address Pettenkoferstr. 9
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
 
Platforms (1)
GPL15053 Applied Biosystems Taqman Array Rodent MicroRNA Cards v2.0
Samples (8)
GSM852552 murine un-diseased arterial tissue on 3 month high fat diet Replicate 1
GSM852553 murine un-diseased arterial tissue on 3 month high fat diet Replicate 2
GSM852554 murine un-diseased arterial tissue on 3 month high fat diet Replicate 3
This SubSeries is part of SuperSeries:
GSE34648 MiRNA expression profile in murine
Relations
BioProject PRJNA156369

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34645_3m_plaque_vs_healthy_ct_raw_data.xlsx.gz 39.8 Kb (ftp)(http) XLSX
Processed data included within Sample table
Raw data are available on Series record

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