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| Status |
Public on Dec 24, 2011 |
| Title |
Gene Expression from diabetic ischemic limbs in WT and p75KO mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptome analysis was carried out to characterise the different responses of diabetic and non-diabetic WT and p75KO mice to ischemia
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| Overall design |
C57BL/6J-p75NTR-/- (p75KO) and p75NTR+/+ (WT) littermates were bred starting from breeding pairs. Six to seven week old male p75KO and WT mice were made diabetic using streptozotocin (STZ, 40 mg/kg in 0.1mol/l citrate buffer pH4.5 i.p. per day for 5 days or left normoglycemic by STZ buffer injections. One or three months after diabetes induction, anesthetized mice underwent unilateral hindlimb ischemia by permanent ligation and electro-coagulation of the proximal end of femoral arteries. At the same time, full thickness wounds were created in the thigh dorsal skin of both legs using a sterile 5-mm-wide biopsy punch. Samples for gene expression analysis were taken from the wounds three days later.
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| Contributor(s) |
Vierlinger K, Emanueli C |
| Citation(s) |
23065828 |
| Submission date |
Dec 22, 2011 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Klemens Vierlinger |
| E-mail(s) |
klemens.vierlinger@ait.ac.at
|
| Organization name |
AIT
|
| Department |
HMD
|
| Street address |
Giefinggasse 4
|
| City |
Vienna |
| ZIP/Postal code |
1210 |
| Country |
Austria |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA150321 |
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