|
| Status |
Public on Oct 05, 2012 |
| Title |
Expression data from murine fibroblasts overexpressing Fbxl10 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Posttranslational modifications on histone tails control gene expresssion levels in an either positive or negative way. Therefore enzymes which modifiy the modifications on the histon tails, like histone demethylases, are able to strictly regulate the transcriptom.
To analyse the regulatory role of histone demethylase Fbxl10 we generated a stable cell line permanently overexpressing HA-tagged Fbxl10 and identified the transcriptome changes compared to untransfected control cell line by microarray.
|
| |
|
| Overall design |
Tet-off Mefs were transfected with a plasmid containing HA-Fbxl10 cDNA and a Hygromycin resistence plasmid and cell clones were selected for HA-Fbxl10 expression. Three independent RNA samples were generated from untransfected Tet-off Mef cells (C1-C3) and from Tet-off Mef HA-Fbxl10 (D1-D3) and hybridisated on Affymetrix microarray.
|
| |
|
| Contributor(s) |
Janzer A, Kirfel J |
| Citation(s) |
22825849 |
| Submission date |
Dec 23, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Andreas Janzer |
| E-mail(s) |
andreas.janzer@uni-bonn.de
|
| Organization name |
University of Bonn
|
| Department |
Pathology
|
| Lab |
Kirfel
|
| Street address |
Sigmund Freud St. 25
|
| City |
Bonn |
| ZIP/Postal code |
53127 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA150295 |
Less...
More...