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Series GSE34873 Query DataSets for GSE34873
Status Public on Aug 08, 2012
Title Experimental identification of miR-378-3p targets by overexpression and silencing in murine NIH-3T3 fibroblasts
Organism Mus musculus
Experiment type Expression profiling by array
Summary MicroRNAs (miRNAs) are short noncoding RNA molecules regulating the expression of mRNAs. Target identification of miRNAs is computationally difficult due to the relatively low homology between miRNAs and their targets. We provide data here utilizing an experimental approach to identify targets of mmu-miR-378-3p, where mmu-miR-378-3p was overexpressed and silenced in NIH-3T3 murine fibroblasts and compared to control RNA transfected cells (RISC-free siRNA). Expression of mRNAs was profiled and differentially expressed genes following either treatment as compared to control transfected cells were identified. In this way we identified 491 significantly differentially expressed genes with more than 1.4 fold change in either comparison. One of the putative targets Akt-1 was subsequently confirmed by luciferase reporter assay.
 
Overall design All conditions were assayed in triplicates. A commercially available mimic or inhibitor of mmu-miR-378-3p or control RNA (RISC-free siRNA) were transfected into NIH-3T3 fibroblasts using a chemical transfection system (DharmaFECT 1). 48h post transfection total RNA was isolated and mRNA-expression profiled.
 
Contributor(s) Buck AH, Laqtom NN, Ruckerl DA
Citation(s) 22855601
Submission date Jan 05, 2012
Last update date Mar 04, 2019
Contact name Dominik Andreas Rückerl
E-mail(s) dominik.ruckerl@manchester.ac.uk
Organization name The University of Manchester
Department Faculty of Biology, Medicine and Health
Street address Oxford Road
City Manchester
ZIP/Postal code M13 9PT
Country United Kingdom
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (9)
GSM856798 NIH-3T3_Risc-transfected_48h_rep1
GSM856799 NIH-3T3_Risc-transfected_48h_rep2
GSM856800 NIH-3T3_Risc-transfected_48h_rep3
Relations
BioProject PRJNA150103

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34873_RAW.tar 33.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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