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| Status |
Public on Jun 27, 2012 |
| Title |
Expression data from murine colon tissue after exposure to 4% DSS for 6 days DSS followed by 4 days of water or 1% L-arginine |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
L-Arginine (L-Arg) is the substrate for both inducible nitric oxide synthase and arginase, which are upregulated in human IBD and in mouse colitis models. We have found that L-Arg supplementation enhances wound restitution in vitro, and improves the clinical parameters of weight loss, survival, and colon weight/length, in dextran sulfate sodium (DSS) induced murine colitis. Our aim was to further identify the potential mechanisms underlying the clinical benefit of L-Arg supplementation.
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| Overall design |
12 Total samples were analyzed, 3 samples from each of 4 groups. We generated the following pairwise comparisons: Ctrl vs Ctrl + L-Arg; Ctrl vs DSS; DSS vs DSS + L-Arg; Ctrl + L-Arg vs DSS, Ctrl + L-Arg vs DSS + L-Arg. Genes with a p-value < 0.01 and a fold-change ≥2 were selected. To identify genes that were altered in response to L-Arg, we performed the following multiple sample comparisons using a p-value < 0.01 and a fold-change ≥2: Ctrl vs DSS vs DSS + L-Arg
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| Contributor(s) |
Wilson KT, Coburn LA, Zhao Z, Gong X |
| Citation(s) |
22428068 |
| Submission date |
Jan 05, 2012 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Keith Wilson |
| E-mail(s) |
keith.wilson@vanderbilt.edu
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| URL |
http://
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| Organization name |
Vanderbilt University School of Medicine
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| Street address |
2215B Garland Ave.
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| City |
Nashville |
| ZIP/Postal code |
TN 37232-0252 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM856807 |
control colon tissue, biological rep1 |
| GSM856808 |
control colon tissue, biological rep2 |
| GSM856809 |
control colon tissue, biological rep3 |
| GSM856810 |
control + L-Arg colon tissue, biological rep1 |
| GSM856811 |
control + L-Arg colon tissue, biological rep2 |
| GSM856812 |
control + L-Arg colon tissue, biological rep3 |
| GSM856813 |
DSS colon tissue, biological rep1 |
| GSM856814 |
DSS colon tissue, biological rep2 |
| GSM856815 |
DSS colon tissue, biological rep3 |
| GSM856816 |
DSS + L-Arg colon tissue, biological rep1 |
| GSM856817 |
DSS + L-Arg colon tissue, biological rep2 |
| GSM856818 |
DSS + L-Arg colon tissue, biological rep3 |
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| Relations |
| BioProject |
PRJNA150105 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34874_RAW.tar |
50.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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