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Status |
Public on Apr 26, 2012 |
Title |
Expression data from mouse lungs during E. coli pneumonia in the presence or absence of LIF neutralization |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Leukemia inhibitory factor (LIF) is amongst the IL-6 family cytokines expressed in the lungs during pneumonia. However, the function of endogenous LIF during pneumonia has never been explored. The purpose of this study was to determine the transcriptional response to pneumonia in the lungs and whether or how this response is influenced by LIF. Mice received intratracheal instillations of vehicle (PBS) or Escherichia coli (10^6 CFU). Control IgG or anti-LIF specific IgG was included in the instillate. 24 hours after the challenge, lungs were collected for RNA isolation and microarray analysis.
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Overall design |
RNA from 3 separate groups of mice (3 mice per group) was analyzed: 1) 24h Vehicle/control IgG-treated; 2) 24h E. coli/control IgG-treated; and 3) 24h E. coli/anti-LIF-specific IgG.
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Contributor(s) |
Quinton LJ |
Citation(s) |
22581855 |
Submission date |
Jan 06, 2012 |
Last update date |
May 17, 2012 |
Contact name |
Lee Quinton |
Organization name |
Boston University School of Medicine
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Street address |
72 E Concord St
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02118 |
Country |
USA |
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Platforms (1) |
GPL11078 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: MoGene10stv1_Mm_ENTREZG_13] |
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Samples (9)
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GSM857302 |
Uninfected control IgG-treated lungs, biological rep1 |
GSM857303 |
Uninfected control IgG-treated lungs, biological rep2 |
GSM857304 |
Uninfected control IgG-treated lungs, biological rep3 |
GSM857305 |
24h E.coli-infected, control IgG-treated lungs, biological rep1 |
GSM857306 |
24h E.coli-infected, control IgG-treated lungs, biological rep2 |
GSM857307 |
24h E.coli-infected, control IgG-treated lungs, biological rep3 |
GSM857308 |
24h E.coli-infected, anti-LIF-treated lungs, biological rep1 |
GSM857309 |
24h E.coli-infected, anti-LIF-treated lungs, biological rep2 |
GSM857310 |
24h E.coli-infected, anti-LIF-treated lungs, biological rep3 |
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Relations |
BioProject |
PRJNA150175 |
Supplementary file |
Size |
Download |
File type/resource |
GSE34901_RAW.tar |
53.9 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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