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Series GSE35047 Query DataSets for GSE35047
Status Public on Aug 08, 2012
Title MicroRNA expression in thioglycollate and alternatively activated
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; Human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Human polyomavirus 1; Macaca mulatta polyomavirus 1
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary Macrophage activation must be tightly controlled to prevent overzealous responses that cause self-damage. MicroRNAs have been shown to promote classical macrophage activation by blocking concomitant anti-inflammatory signals and transcription factors, but can also place restraints on activation by preventing excessive TLR-signalling. In contrast, the microRNA profile associated with alternatively activated macrophages and their role in regulating wound-healing or anti-helminthic responses has not yet been described. Utilizing an in vivo model of alternative activation, in which adult Brugia malayi nematodes are surgically implanted in the peritoneal cavity of mice, we examined the profile of microRNA expression in these alternatively activated macrophages and compared this to alternatively activated IL-4 receptor knockout macrophages and thioglycollate elicited macrophages.
 
Overall design Peritoneal macrophages from BALB/c wild type or IL-4 receptor knockout mice were elicited with thioglycollate or using nemtodes (peritoneal implant of Brugia malayi). The latter leads to a population of alternatively activated macrophages. Microarray analysis was used to examine the microRNA profile of WT alternatively activated macrophages (n = 4), IL-4 receptor knockout alternatively activated macrophages (n = 4), WT thioglycollate elicited macrophages (n = 3) and IL-4 receptor knockout thioglycollate elicited macrophages (n = 3).
 
Contributor(s) Gallagher IJ
Citation(s) 22855601
Submission date Jan 12, 2012
Last update date Aug 08, 2012
Contact name Iain Gallagher
E-mail(s) iaingallagher@gmail.com
Phone 00 44 1786 46 6024
Organization name University of Stirling
Department Faculty of Health Sciences and Sport
Street address Room 4B133 Cottrell Building, University of Stirling, Airthrey Road
City Stirling
ZIP/Postal code FK9 4LA
Country United Kingdom
 
Platforms (1)
GPL15121 EXIQON miRCURY™ V8.1 LNA array [miRbase 12]
Samples (14)
GSM861288 thiowt_1 signal
GSM861289 thiowt_2 signal
GSM861290 thiowt_3 signal
Relations
BioProject PRJNA150857

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35047_slide10.txt.gz 548.8 Kb (ftp)(http) TXT
GSE35047_slide12.txt.gz 553.5 Kb (ftp)(http) TXT
GSE35047_slide14.txt.gz 551.9 Kb (ftp)(http) TXT
GSE35047_slide15.txt.gz 558.1 Kb (ftp)(http) TXT
GSE35047_slide6.txt.gz 548.6 Kb (ftp)(http) TXT
GSE35047_slide7.txt.gz 550.4 Kb (ftp)(http) TXT
GSE35047_slide9.txt.gz 554.6 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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