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| Status |
Public on Oct 27, 2005 |
| Title |
Mutational state FLT3-ITD and GATA2 overexpression could define a subgroup of AML-M1 patients with normal karyotype |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
More than 40% of patients with AML have a normal karyotype and are included in the intermediate prognostic group, in which risk classification is currently poor defined and more molecular markers are needed to achieve treatment stratification. EVI1 overexpression (OE) has been reported to discriminate in this group those with a worse prognosis. The EVI1 mice homolog has a role in the HSC proliferation through Gata2 expression, therefore, GATA2, a transcription factor with a relevant role in hematopoiesis, could be a candidate gene in the leukemogenic transformation in AML in patients with normal karyotype, and in other subgroups. GATA2 OE was detected in 46% of cases with normal karyotype, and was more frequent among samples with FLT3-ITD (p=0.0021), especially among the AML-M1 cases. We found a mutational pattern FLT3-ITD/GATA2-OE/WT1-OE that could define a subgroup of patients with normal karyotype and AML-M1, with a different gene expression array pattern and a poor prognosis. Our results show that GATA2 OE is a common event in AML cases with normal karyotype (46%). The deregulation of the expression of the GATA2 transcription factor would lead to a hematopoietic differentiation impairs, focusing GATA2 as a candidate gene that fits in the cooperating model for the multistep pathogenesis that causes AML transformation.
Keywords: Disease state analysis
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| Overall design |
Nine slides were hybridized, one for each patient, being three patients per group. Each patient sample is labeled with cy5 and the reference sample, the same for all slides, is a pool of nine bone marrows from healthy patients, labeled with cy3.
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| Contributor(s) |
Lahortiga I, Bandrés E, Malumbres R, Odero MD |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 25, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Raquel Malumbres |
| E-mail(s) |
rmalumbres@yahoo.com
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| Phone |
+34 948194700
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| Organization name |
University of Navarra/ Clínica Universidad de Navarra
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| Department |
Hemato-Oncology
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| Lab |
Multiple Myeloma
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| Street address |
Avenida Pio XII 55
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| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
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| Platforms (1) |
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| Samples (9)
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| GSM79782 |
14413, GATA2 overexpresed, FLT3 non-mutated |
| GSM79783 |
20505, GATA2 overexpresed, FLT3 non-mutated |
| GSM79992 |
23353, GATA2 overexpresed, FLT3 non-mutated |
| GSM79993 |
16320, GATA2 non-overexpresed, FLT3 non-mutated |
| GSM80023 |
18861, GATA2 non-overexpresed, FLT3 non-mutated |
| GSM80024 |
26611, GATA2 non-overexpresed, FLT3 non-mutated |
| GSM80025 |
16583, GATA2 overexpresed, FLT3 mutated |
| GSM80026 |
18788, GATA2 overexpresed, FLT3 mutated |
| GSM80027 |
20531, GATA2 overexpresed, FLT3 mutated |
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| Relations |
| BioProject |
PRJNA93339 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE3505_RAW.tar |
17.5 Mb |
(http)(custom) |
TAR (of TXT) |
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