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Series GSE35142 Query DataSets for GSE35142
Status Public on Dec 20, 2013
Title Transcriptional responses to engineered nanoplatforms for drug delivery in primary human aortic endothelial cells.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This study evaluated transcriptional effects of nanomaterials that have been proposed for use as platforms for drug delivery. We tested SiO2 that had been surface modified to have a positive zetapotential of different geometries as well PAMAM dendrimers with different surface charges. We tested these materials on human aortic endothelial cells (HAECs) since we were interested in determining if there was a toxicogenomic response in endothelial cells that may come into contact with drug delivery nanoplatforms.
The most pronounced transcriptional response resulted from the SiO2 treatement - the most prevelant responses were cell cycle, lipid metabolism and pro-inflammatory responses - with fewer responses from the PAMAM dendrimers. The lipid metabolism responses may relate to teh positive surface character as this response was not observed in the G3.5-COOH dendrimers. Indeed, the G3.5-COOH dendrimers were non-toxic and did not demonstrate any consistent transcriptional response.
 
Overall design Primary human aortic endothial cells (HAECs) were grown in 6 well plates (in 2 ml of medium) until they were >80% confluent by visual inspection. Daily media changes allowed continued growth for HAECs that demonstrate contact inhibition. The cells were then incubated with nanomaterials: surface modified SiO2 with worm and sphere goemetries (the spheres were 200 nm in diameter and the worm's cylindrical diameter 200 nm and the length was ~ 1000 nm); and PAMAM dendrimers with different surface charges (G3.5-COOH are negative and G4-NH2 are positive). RNA was collected after 4 and 24 hrs (one SiO2 worm sample was at 1.5 hrs due to space available on the 4-pack microarrays).
Triplicate biological samples (indicated by the 'a', 'b', and 'c' designations in the sample names) were evaluated for gene expression changes by microarray analysis.
 
Contributor(s) Moos PJ
Citation(s) 23806026
Submission date Jan 17, 2012
Last update date Feb 22, 2018
Contact name Philip J Moos
E-mail(s) philip.moos@utah.edu
Phone 801-585-5952
Organization name University of Utah
Department Pharmacology & Toxicology
Lab Moos
Street address 30 S 2000 East, Rm 201
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (28)
GSM862576 200 nm SiO2 worms 4 hr incubation 100 ug/ml nSiO2_W4a
GSM862577 200 nm SiO2 worms 4 hr incubation 100 ug/ml nSiO2_W4b
GSM862578 200 nm SiO2 worms 4 hr incubation 100 ug/ml nSiO2_W4c
Relations
BioProject PRJNA150707

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35142_RAW.tar 440.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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