|
| Status |
Public on Mar 31, 2009 |
| Title |
A compact VEGF signature associated with distant metastases and poor outcomes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Results
When compared with each other, primary tumors and regional metastases showed statistically indistinguishable gene expression patterns. Supervised analyses comparing patients with distant metastases versus primary tumors or regional metastases showed
that the distant metastases were distinct and distinguished by the lack of expression of fibroblast/mesenchymal genes, and by the high expression of a 13-gene profile (that is, the ‘vascular endothelial growth factor (VEGF) profile’) that included VEGF, ANGPTL4, ADM and the monocarboxylic acid transporter SLC16A3. At least 8 out of 13 of these
genes contained HIF1α binding sites, many are known to be HIF1α-regulated, and expression of the VEGF profile correlated with HIF1α IHC positivity. The VEGF profile also showed prognostic significance on tests of sets of patients with breast and lung cancer and glioblastomas, and was an independent predictor of outcomes in primary breast cancers when tested in models that contained other prognostic gene expression
profiles and clinical variables.
Conclusions
These data identify a compact in vivo hypoxia signature that tends to be present in distant metastasis samples, and which portends a poor outcome in multiple tumor types.
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| |
|
| Overall design |
Microarrays and immunohistochemistry were used to analyze primary breast tumors, regional (lymph node) metastases, and distant metastases in order to identify biological features associated with distant metastases.
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| |
|
| Contributor(s) |
Hu Z, Perou CM |
| Citation(s) |
19291283 |
| Submission date |
Oct 27, 2005 |
| Last update date |
Feb 20, 2017 |
| Contact name |
Charles M. Perou |
| E-mail(s) |
cperou@med.unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Professor of Genetics, and Pathology & Laboratory Medicine; Lineberger Comprehensive Cancer Center
|
| Street address |
12-044 Lineberger Comprehensive Cancer Center CB# 7295
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7264 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL885 |
Agilent-011521 Human 1A Microarray G4110A (Feature Number version) |
| GPL887 |
Agilent-012097 Human 1A Microarray (V2) G4110B (Feature Number version) |
| GPL1390 |
Agilent Human 1A Oligo UNC custom Microarrays |
|
| Samples (195)
|
|
| Relations |
| BioProject |
PRJNA93569 |
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