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Series GSE35232 Query DataSets for GSE35232
Status Public on Oct 29, 2013
Title Transcription profile of adipose derived mesenchymal stem cells separated by cell surface protein N-cadherin.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Mesenchymal stem cells (MSCs) are one of most promising stem cell sources for regenerative medicine. MSCs have a differention ability into several tissues. However, their differentiation efficiency is considered quite limited. Especially, the differentiation efficiency into cardiomyocytes is very low. N-cadherin is one of the cell surface protein that is expressed in developing and adult cardiomyocytes. We found that cell surface expression of N-cadherin in MSCs shows good correlation with the cardiomyogenic differentiation ability. We also analyzed the expression profiles of N-cadherin-positive and N-cadherin-negative fraction by microarray. We found that N-cadherin-positive MSCs show higher expression of some of the cardiomyogenesis-related genes than N-cadherin-negative MSCs.
 
Overall design The ASCs were maintained in MesenPRO RSTM Medium. The ASCs were harvested in a cell dissociation buffer. The ASCs were incubated with biotinylated anti N-cadherin antibody and next incubated with streptavidin –APC. Subsequently cells were incubated with anti-APC microbeads. Immunomagnetic separation of N-cadherin+ cells were performed with autoMACSTMPro separator applying the separation program ‘depl025’
 
Contributor(s) Ishimine H, Kurisaki A, Asashima M
Citation(s) 23899519
Submission date Jan 20, 2012
Last update date Jan 23, 2019
Contact name Hisako Ishimine
Organization name National Institute of Advanced Industrial Science and Technology
Department Research Center for Stem Cell Engineering
Street address higashi1-1-1 Tsukuba central 4
City Tsukuba
State/province Japan Ibaraki
ZIP/Postal code 305-8562
Country Japan
 
Platforms (1)
GPL6480 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
Samples (3)
GSM864299 ASC_N-cadherin(+)_rep1
GSM864300 ASC_N-cadherin(-)_rep1
GSM864301 ASC_rep1
Relations
BioProject PRJNA152859

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35232_RAW.tar 6.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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