|
| Status |
Public on Dec 01, 2012 |
| Title |
Genistein Cooperates with the Histone Deacetylase Inhibitor Vorinostat to Induce Cell Death in Prostate Cancer Cells (methylation data) |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
|
| Summary |
We have investigated the effects of genistein on several prostate cancer cell lines, including the ARCaP-E/ARCaP-M model of the epithelial-to-mesenchymal transition (EMT), to analyze effects on their epigenetic state. In addition, we investigated the effects of combined treatment of genistein with the histone deacetylase inhibitor vorinostat on survival in prostate cancer cells. Using whole-genome expression profiling and whole-genome methylation profiling, we have determined the genome-wide differences in genetic and epigenetic responses to genistein in prostate cancer cells before and after undergoing the EMT. Also, cells were treated with genistein, vorinostat, and a combination treatment, where cell death and cell proliferation was determined.
|
| |
|
| Overall design |
ARCAP-E, ARCAP-M, and normal human PrEC cells were analyzed for genome-wide methylation using the Illumina 27K CpG Methylation BeadChip. ARCAP-E and ARCAP-M cells were treated with DMSO as a negative control, genistein, or 5-aza-deoxycytidine as a positive control for demethylation. PrEC cells, used as a normal human prostate cell line control, were untreated.
|
| |
|
| Contributor(s) |
Phillip CJ, Giardina CK, Lai YH, Kucuk O, Moreno CS |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 20, 2012 |
| Last update date |
Jan 02, 2015 |
| Contact name |
Khanjan Gandhi |
| E-mail(s) |
khanjan.gandhi@emory.edu
|
| Organization name |
Winship Cancer Institute, Emory University.
|
| Street address |
1365-B Clifton Rd NE, Room 4107
|
| City |
Atlanta |
| State/province |
Georgia |
| ZIP/Postal code |
30363 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL8490 |
Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2) |
|
| Samples (22)
|
|
| This SubSeries is part of SuperSeries: |
| GSE35246 |
Genistein Cooperates with the Histone Deacetylase Inhibitor Vorinostat to Induce Cell Death in Prostate Cancer Cells |
|
| Relations |
| BioProject |
PRJNA156033 |
Less...
More...