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Series GSE35291 Query DataSets for GSE35291
Status Public on Jan 24, 2012
Title Expression profiles of 7-day cultured HSPCs in the presence or absence of valproic acid and/or lithium
Organism Mus musculus
Experiment type Expression profiling by array
Summary Two small molecules, valproic acid (VPA) and lithium (Li), were tested to inhibit differentiation of hematopoietic stem/progenitor (HSPC) cells in culture. HSPCs exposed to VPA and Li during differentiation-inducing culture preserved an immature cell phenotype, provided radioprotection to lethally irradiated recipients and enhanced in vivo repopulating potential. Furthermore, VPA and Li synergistically preserved expression of stem cell-related genes and repressed genes involved in differentiation. Target genes were collectively co-regulated during normal hematopoietic differentiation. Additionally, transcription factor networks were identified as possible primary regulators. Our results demonstrate that the combination of VPA and Li potently prevents differentiation at the biological and the molecular level, and provide evidence to suggest that combinatorial screening of chemical compounds may uncover possible additive/synergistic effects to modulate stem cell fate decisions.
 
Overall design These data consist of total mRNA obtained from hematopoietic cells cultured for 7 days in the presence or absence of valproic acid and/or lithium. All samples were analyzed in independent biological triplicates.
 
Contributor(s) Walasek MA, Bystrykh LV, van den Boom V, Olthof S, Ausema A, Ritsema M, Huls G, de Haan G, van Os R
Citation(s) 22327222
Submission date Jan 24, 2012
Last update date Jun 14, 2018
Contact name Leonid Bystrykh
E-mail(s) l.bystrykh@umcg.nl
Organization name University Medical Center Groningen
Department European Research Institute for the Biology of Ageing - ERIBA
Lab Ageing Biology and Stem Cells
Street address A. Deusinglaan 1
City Groningen
State/province Groningen
ZIP/Postal code 9713 AV
Country Netherlands
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (12)
GSM841634 Growth_factors_only_rep1
GSM841635 Growth_factors_only_rep2
GSM841636 Growth_factors_only_rep3
This SubSeries is part of SuperSeries:
GSE34088 The combination of valproic acid and lithium prevents hematopoietic stem/progenitor cell differentiation.
Relations
BioProject PRJNA156021

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35291_M.Walasek_Raw_Data_Sample_Probe_Profile.txt.gz 7.0 Mb (ftp)(http) TXT
GSE35291_RAW.tar 3.1 Mb (http)(custom) TAR
GSE35291_non_normalized.txt.gz 1.9 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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