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Series GSE35301 Query DataSets for GSE35301
Status Public on Apr 11, 2013
Title Total Gene expression analysis of H3f3b conditional knockout MEFs
Organism Mus musculus
Experiment type Expression profiling by array
Summary Total gene expression analysis was performed on CRE induced conditional knockout E12.5 MEFs relative to GFP infected control MEFs. Intent was to analyze the role of H3f3b in overall gene expression.
 
Overall design For conditional KO, three lines of H3f3b FL/FL E12.5 MEFs (cKO1, cKO2 and pcKO1) were transduced with Cre retroviruses and compared to their respective lines of MEFs transduced solely with GFP vector (as control). Total RNA was isolated for gene analysis.
 
Contributor(s) Bush KM, Knoepfler P, Riggs JW
Citation(s) 23570311
Submission date Jan 24, 2012
Last update date Jun 14, 2018
Contact name Paul Knoepfler
E-mail(s) knoepfler@ucdavis.edu
Phone 916-453-2289
Organization name UC Davis
Department Cell Biology and Human Anatomy
Lab Knoepfler Lab
Street address 2425 Stockton Blvd
City Sacramento
State/province CA
ZIP/Postal code 95817
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM865479 H3f3b cKO1 + IRES-GFP
GSM865480 H3f3b cKO1 + CRE-IRES-GFP
GSM865481 H3f3b cKO2 + IRES-GFP
This SubSeries is part of SuperSeries:
GSE35321 Gene expression changes with loss of H3f3b
Relations
BioProject PRJNA156025

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35301_RAW.tar 3.1 Mb (http)(custom) TAR
GSE35301_non-normalized.txt.gz 913.2 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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