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| Status |
Public on Mar 06, 2007 |
| Title |
Role of DNA methylation in stable gene repression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The experiment was designed to study the effects of the Dnmt1 gene knockout on gene expression, as well as to analyze the effects of TSA treatment on both WT (p53-/-) and knockout (p53-/-Dnmt1-/-) cells
The effect of TSA treatment on knockout and WT cells was tested at two different time points (24 and 48 hs), as well as two weeks after removal of TSA
Keywords: Effect of demetyhlation and/or reacetylation on gene expression
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| Overall design |
Seven hybridizations were performed: 1. p53-/- untreated (control), 2. p53-/- +TSA harvested after 24hs of exposure to TSA, 3. p53-/- +TSA 48hs, 4. p53-/-Dnmt1-/- untreated, 5.p53-/-Dnmt1-/- +TSA 24hs 6.p53-/-Dnmt1-/- +TSA 48hs 7.p53-/-Dnmt1-/- two weeks after removal of TSA
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| Contributor(s) |
Lande-Diner L, Zhang J, Ben-Porath I, Amariglio N, Keshet I, Azuara V, Fisher AG, Rechavi G, Cedar H |
| Citation(s) |
17311920 |
| Submission date |
Oct 30, 2005 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Laura Lande-Diner |
| Organization name |
Hebrew University Medical School
|
| Department |
Cell Biochemistry and Human Genetics
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| Lab |
Cedar
|
| Street address |
Ein Kerem
|
| City |
Jerusalem |
| ZIP/Postal code |
91120 |
| Country |
Israel |
| |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (7)
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| GSM80305 |
p53-/- Dnmt1-/- untreated (control) |
| GSM80306 |
p53-/- Dnmt1-/- TSA 24 |
| GSM80307 |
p53-/- Dnmt1-/- TSA 48 |
| GSM80308 |
p53-/- and Dnmt1-/- two weeks after TSA removal |
|
| Relations |
| BioProject |
PRJNA93589 |
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