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Series GSE35373 Query DataSets for GSE35373
Status Public on Jan 28, 2012
Title Reprogramming of prostate cancer-associated stromal cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary CD90+ prostate cancer-associated (CP) stromal cells represent a disease cell type found only in tumor tissue. Genetic reprogramming by induced pluripotent stem (iPS) cell technology might be used to “normal gene expression of diseased cells thereby providing a cure. The resultant iPS cells would no longer express the disease program, and, like stem cells, might respond to normal differentiative signaling. Thus, CP stromal cells, isolated from tumor tissue and cultured in vitro, were transfected with POU5F1/LIN28/NANOG/SOX2 lentiviral vectors. iPS cells were obtained at a frequency of 10^4. Transcriptome analysis showed an almost complete match in gene expression between the iPS cells and human embryonic stem cells. Genes of CP stromal cells were fully inactivated.
 
Overall design CP stromal cells were isolated from tissue, cultured and transfected with lentiviral vectors containing NANOG, SOX2, POU5F1, LIN28. The resultant induced pluripotent cells were analyzed by DNA microarrays.
 
Contributor(s) Liu AY
Citation(s) 22314551
Submission date Jan 27, 2012
Last update date Mar 25, 2019
Contact name Alvin Y Liu
E-mail(s) aliu@uw.edu
Phone 206 221-0603
Fax 206 543-2275
Organization name University of Washington
Department Urology
Street address 815 Mercer Street Box 358056
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM867091 Embryonal carcinoma cell line NCCIT
GSM867092 CP stromal cells, sorted 1
GSM867093 CP stromal cells, cultured
Relations
BioProject PRJNA152605

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Supplementary file Size Download File type/resource
GSE35373_RAW.tar 29.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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