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| Status |
Public on Oct 10, 2012 |
| Title |
Expression data from normal melanocyte, melanoma cells and their exosomes (microRNA) |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Exosomes are small membraneous vesicles secreted into body fluids by tumors. Tumor exosomes contain intact and functional mRNAs, small RNAs (including miRNAs), and proteins that can alter the cellular environment to favor tumor growth. Further exploration into the molecular profiling of exosomes may increase our understanding of their roles in melanoma progression in vivo, and may have potential application in biomarker studies. In the present study, we used mRNA array profiling to identify thousands of exosomal mRNAs associated with melanoma progression and metastasis. Similarly, miRNA array profiling identified specific miRNAs, such as hsa-miR-31, -185, and -34b, involved in melanoma invasion. Our results indicate that melanoma-derived exosomes have unique gene expression signatures and miRNA profiles that may have important functions in melanoma metastasis and progression.
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| Overall design |
Total RNA from cells and exosomes were isolated using mirVana total RNA isolation kit according to the manufacturer’s guidelines. RNA was quantified using Nanodrop ND-1000. The integrity of these total RNAs was assessed using Agilent 2100 Bioanalyzer. Total high-quality RNA was labelled. The miRNA array profiling was performed by using the Affymetrix GeneChip miRNA Array 1.0. Two different RNA preparations from two cell lines and their exosomes were used, except that only one RNA preparation was used for HEMa-LP exosome miRNA array. Due to the limited number of passages (approximately 10), adequate exosomal RNA and proteins from HEMa-LP cells for multiple analyses was not available.
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| Contributor(s) |
Xiao D, Ohlendorf J, Chen Y, Taylor DD, Rai S, Waigel S, Zacharias W, Hao H, McMasters KM |
| Citation(s) |
23056502 |
| Submission date |
Jan 27, 2012 |
| Last update date |
May 02, 2017 |
| Contact name |
Hongying Hao |
| E-mail(s) |
h0hao001@louisville.edu
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| Phone |
502-852-8404
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| Organization name |
University of Louisville
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| Department |
Surgery
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| Lab |
Dr. Kelly M McMasters
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| Street address |
505 South Hancock Street
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| City |
Louisville |
| State/province |
KY |
| ZIP/Postal code |
40202 |
| Country |
USA |
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| Platforms (1) |
| GPL8786 |
[miRNA-1] Affymetrix Multispecies miRNA-1 Array |
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| Samples (7)
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| GSM867220 |
melanoma cell A375, microRNA biological rep 1 |
| GSM867221 |
melanoma cell A375, microRNA biological rep2 |
| GSM867222 |
melanoma exosome A375, microRNA biological rep 1 |
| GSM867223 |
melanoma exosome A375, microRNA biological rep 2 |
| GSM867224 |
normal melanocyte HEMa-LP, microRNA biological rep 1 |
| GSM867225 |
normal melanocyte HEMa-LP, microRNA biological rep 2 |
| GSM867226 |
normal melanocyte HEMa-LP, microRNA exosome |
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| This SubSeries is part of SuperSeries: |
| GSE35389 |
Expression data from normal melanocyte, melanoma cells and their exosomes |
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| Relations |
| BioProject |
PRJNA156069 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE35387_RAW.tar |
1.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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