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Series GSE35398 Query DataSets for GSE35398
Status Public on Mar 30, 2012
Title Expression data from MDSCs at different time points during tumor progression
Organism Mus musculus
Experiment type Expression profiling by array
Summary Myeloid-derived suppressor cells (MDSCs) are increased by tumor-derived factors and suppress anti-tumor immunity. MDSCs obtained at a late time point after tumor injection had stronger suppressive activity than MDSCs obtained at an early time point, as measured by T cell proliferation assays. To find factors in MDSCs that change during tumor growth, we analyzed gene expression profiles from MDSCs at different time points after tumor injection. We found that immune response-related genes were down-regulated, but pro-tumor function-related genes were up-regulated in both Mo-MDSCs and PMN-MDSCs at the late time point. Among differentially expressed genes, FK506 binding protein 51 (FKBP51), which is a member of the immunophilin protein family and plays a role in immunoregulation, was increased in the Mo- and PMN-MDSCs isolated from the late time points. Experiments using siRNA and a chemical inhibitor of FKBP51 revealed that FKBP51 contributes to the regulation of the suppressive function of MDSCs by increasing iNOS, ARG1, and ROS levels and enhancing NF-kappaB activity. Collectively, our data suggest that FKBP51 is a novel molecule that can be targeted to regulate the immunosuppressive function of MDSCs.
 
Overall design To identify the factors that licensed MDSCs to be more suppressive as tumors grow, we analyzed gene expression profiles in the two subsets of MDSCs at different time points (3wks, 6wks) during tumor progression. CD11b+Ly-6C(high)Ly-6G(low) Mo-MDSCs and CD11b+Ly-6C(low)Ly-6G(high) PMN-MDSCs were sorted from pooled spleens of naïve mice and Her-2/CT26 tumor-bearing mice. Total RNA was purified and gene expression was analyzed by the Affymetrix GeneChip® Mouse Gene 1.0 ST Array.
 
Contributor(s) Kim Y, Kim Y, Kang C
Citation(s) 22474024
Submission date Jan 28, 2012
Last update date Mar 04, 2019
Contact name Chang-Yuil Kang
E-mail(s) cykang@snu.ac.kr
Phone 82-2-880-7860
Organization name Seoul National University
Street address 29-102, Seoul National University, Shillim-dong, Gwanak-gu
City Seoul
ZIP/Postal code 151-742
Country South Korea
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (17)
GSM867335 CD11b+L6G-Ly6C+ cells, naïve mice, rep1
GSM867336 CD11b+L6G-Ly6C+ cells, naïve mice, rep2
GSM867337 CD11b+L6G-Ly6C+ cells, naïve mice, rep3
Relations
BioProject PRJNA152783

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35398_RAW.tar 81.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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