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Series GSE35415 Query DataSets for GSE35415
Status Public on Sep 04, 2012
Title UTX regulated genes in mouse embryonic stem cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary UTX gene is localized on the X chromosome, identified as a demethylase on histone H3 lysine 27.
Overall design Two independent UTX conditional knockout (cKO) embryonic stem cell lines and two UTX knockout (KO) cell lines were cultured on 0.1% gelatin-coated dishes without feeder cells at desity of 1 million cells/10 cm dish. Cells were cultured overnight and collected. Total RNAs were sequentially purified with Trizol (Invitrogen) and RNeasy kit (Qiagen) and analyzed on Mouse Genome 430 2.0 Array (Affymetrix) at NIDDK Microarray Core Facility following standard protocols.
Contributor(s) Wang C, Ge K
Citation(s) 22949634
Submission date Jan 30, 2012
Last update date Feb 11, 2019
Contact name Chaochen Wang
Organization name NIDDK
Department LGP
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM867832 UTX conditional knockout (cKO) cell line 1
GSM867833 UTX conditional knockout (cKO) cell line 2
GSM867834 UTX knockout (KO) cell line 1
BioProject PRJNA152573

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35415_RAW.tar 26.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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