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Series GSE35460 Query DataSets for GSE35460
Status Public on Jul 10, 2012
Title Gene expression profiling data from sooty mangabeys treated with interferon alpha
Platform organism Macaca mulatta
Sample organism Cercocebus atys
Experiment type Expression profiling by array
Summary In contrast to pathogenic HIV and SIV infection of humans and macaques, SIV infection of sooty mangabeys (SMs) is typically non-pathogenic despite high virus replication.
A key feature of primary SIV infection of SMs is a strong type I interferon (IFN-I) response, characterized by massive up-regulation of interferon-stimulated genes (ISG), followed by rapid resolution during the acute-to-chronic phase transition and establishment of an immune quiescent state that persists throughout the chronic infection. Based on these observations we hypothesized that low levels of IFN-I signaling may be instrumental in preventing chronic immune activation and disease progression in SIV-infected SMs. We used microarrays to characterize gene expression changes induced by IFNalpha treatment.
 
Overall design To directly assess the effects of an experimentally-induced augmentation of IFN-I signaling in chronically SIV-infected SMs, we administered recombinant rhesus macaque IFNalpha2-IgFc (rmIFNα2) to eight naturally SIV-infected SMs weekly for 16 weeks and longitudinally monitored viral load, lymphocyte counts, immune activation, SIV-specific CD8+ T-cell responses, and gene expression profile. Administration of rmIFNα2 was bioactive in vivo with gene expression profiling revealing a strong upregulation of numerous ISGs in the blood of treated animals.
 
Contributor(s) Vanderford TH, Silvestri G, Bosinger SE, Villinger F, Schlicter C, Lawson BO, Rend O, Tharp GK
Citation(s) 22550346
Submission date Jan 31, 2012
Last update date Jul 18, 2012
Contact name Gregory K Tharp
E-mail(s) gktharp@emory.edu
Phone 404-727-7797
Organization name Yerkes National Primate Research Center
Department Developmental and Cognitive Neuroscience
Lab Genomics Core
Street address 954 Gatewood Dr
City Atlanta
State/province GA
ZIP/Postal code 30329-4208
Country USA
 
Platforms (1)
GPL3535 [Rhesus] Affymetrix Rhesus Macaque Genome Array
Samples (47)
GSM868941 Blood Baseline day -21 Animal ID FYM
GSM868942 Blood IFNalpha Tx day 7 Animal ID FYM
GSM868943 Blood IFNalpha Tx day 21 Animal ID FYM
Relations
BioProject PRJNA152333

Download family Format
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Supplementary file Size Download File type/resource
GSE35460_RAW.tar 209.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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