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| Status |
Public on Jan 24, 2015 |
| Title |
CD98 marks a subpopulation of head and neck squamous cell carcinoma cells with stem cell properties |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The application of compounds specifically targeting the cancer stem cells (CSCs), in addition to routinely used therapeutics, would likely improve the clinical outcome of head and neck squamous cell carcinoma (HNSCC). The previously described monoclonal antibody K984 (now found to recognize CD98) was used to identify, select and isolate a specific tumor subpopulation. These cells were molecularly characterized and subjected to serial transplantation experiments to determine the CSC properties. We show that CD98high cells, in contrast to CD98low cells, are able to generate tumors in immunodeficient mice and that the CD98high subpopulation expresses high levels of cell cycle control and DNA repair genes, while the CD98low fraction shows expression patterns that represent the more differentiated cells forming the bulk of the tumor.
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| Overall design |
Transcriptional profiling of CD98high cells (n=4 fractions) of VU-SCC-OE xenograft tumors compared to CD98low cells (n=3 fractions) from the corresponding tumors.
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| Contributor(s) |
Martens-de Kemp SR, Brink A, Stigter-van Walsum M, Damen JM, Rustenburg F, Wu T, van Wieringen WN, Schuurhuis GJ, Braakhuis BJ, Slijper M, Brakenhoff RH |
| Citation(s) |
23523931 |
| Submission date |
Feb 01, 2012 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Daoud Sie |
| E-mail(s) |
d.sie@vumc.nl
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| Phone |
+31 20 4442428
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| Organization name |
Vrije Universiteit Medical Center
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| Department |
Pathology
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| Lab |
Microarray Core Facility
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| Street address |
De Boelelaan 1117
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| City |
Amsterdam |
| ZIP/Postal code |
1081 HV |
| Country |
Netherlands |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (8)
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| GSM869219 |
Cell line VU-SCC-OE: CD98high fraction tumor 1 |
| GSM869220 |
Cell line VU-SCC-OE: CD98low fraction tumor 1, rep1 |
| GSM869221 |
Cell line VU-SCC-OE: CD98high fraction tumor 2 |
| GSM869222 |
Cell line VU-SCC-OE: CD98low fraction tumor 1, rep2 |
| GSM869223 |
Cell line VU-SCC-OE: CD98low fraction tumor 3 |
| GSM869224 |
Cell line VU-SCC-OE: CD98high fraction tumor 3 |
| GSM869225 |
Cell line VU-SCC-OE: CD98low fraction tumor 4 |
| GSM869226 |
Cell line VU-SCC-OE: CD98high fraction tumor 4 |
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| Relations |
| BioProject |
PRJNA152361 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE35477_RAW.tar |
131.8 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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