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| Status |
Public on Feb 01, 2012 |
| Title |
Comparison of gene expression profiles in NOD and Idd9 CD4+ T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Nonobese diabetic (NOD) mice congenic for B10 derived genes in the Idd9 region of chromosome 4 are highly protected from the development of diabetes. In order to identify possible candidate genes within the Idd9 susceptibility region and possible downstream mediators of protection, we compared gene expression between NOD and Idd9 CD4+ T-cells. CD4+ T-cells with an activated phenotype (CD44-high/CD62L-low) were sorted from the pancreatic lymph nodes of female 8-week old mice.
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| Overall design |
Mice were bred at Taconic farms and were line 905 (NOD.B10-Idd9 congenic) and line 6359 (NOD-like control strain with a B10 congenic region on chromosome 1 which does not modify diabetes incidence).
Three replicate samples were prepared, each from four pooled pancreatic lymph nodes. CD4+CD44-highCD62L-low T-cells were sorted from each sample.
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| Contributor(s) |
Hamilton-Williams EE, Rainbow DB, Cheung J, Wicker LS, Sherman LA, Head SR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 01, 2012 |
| Last update date |
Mar 06, 2018 |
| Contact name |
Steven Head |
| E-mail(s) |
shead@scripps.edu
|
| Phone |
858 784-2263
|
| Organization name |
The Scripps Research Institute
|
| Street address |
10550 N Torrey Pines Rd
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
|
| GSM869274 |
Idd9 mouse pancreatic lymph node rep1 |
| GSM869275 |
Idd9 mouse pancreatic lymph node rep2 |
| GSM869276 |
Idd9 mouse pancreatic lymph node rep3 |
| GSM869277 |
NOD mouse pancreatic lymph node rep1 |
| GSM869278 |
NOD mouse pancreatic lymph node rep2 |
| GSM869279 |
NOD mouse pancreatic lymph node rep3 |
|
| Relations |
| BioProject |
PRJNA152371 |
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