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| Status |
Public on Feb 06, 2012 |
| Title |
Gene-expression profiling of ZNF217-overexpressing MDA-MB-231 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To obtain an overview of the cellular functions regulated by ZNF217 signaling in breast-cancer cell lines, we performed global gene-expression profiling on MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 cells
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| Overall design |
Two independent cell-culture replicates from the total population of MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 stable transfectants were used to generate total RNA. Total RNA was extracted from cell culture using a Qiagen RNA extraction kit and RNA quality was assessed using the BioAnalyzer 2100™ (Agilent Technologies). Complex probes were produced from these RNA, then hybridized to Human genome U133 Plus 2.0 array according to the manufacturer’s recommendations (Affymetrix).
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| |
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| Contributor(s) |
Cohen PA |
| Citation(s) |
22593193 |
| Submission date |
Feb 02, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Julie A Vendrell |
| E-mail(s) |
julie.vendrell@univ-lyon1.fr
|
| Organization name |
ISPBL - Faculté de Pharmacie de Lyon
|
| Department |
INSERM U1052
|
| Lab |
Centre de Recherche en Cancérologie de Lyon
|
| Street address |
8 avenue Rockefeller
|
| City |
Lyon |
| State/province |
Select a State or Province |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA152321 |
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