|
| Status |
Public on Jan 01, 2018 |
| Title |
Analysis of small RNA by microarray in the cell cycle |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Dicer plays a key role in RNA silencing. By processing pre-miRNAs and dsRNAs, Dicer generates miRNAs and siRNAs that act as post-transcriptional regulators of gene expression. Dicer is also implicated in heterochromatin formation and toxic RNA degradation. Here we report that Dicer is controlled in cell cycle. The Dicer protein level drastically rises at late G1 phase and falls at early S phase. Interestingly, the protein stability of Dicer is decreased specifically at early S phase. MG132, an inhibitor of proteasome, increases Dicer protein level, suggesting that the stability of Dicer is controlled via ubiquitination-dependent proteasome pathway.
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| |
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| Overall design |
Examination of small RNA regulation in the cell cycle
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| |
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| Contributor(s) |
Lee JH, Chang H, You K, Kim VN |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 03, 2012 |
| Last update date |
Jan 01, 2018 |
| Contact name |
Hyeshik Chang |
| E-mail(s) |
hyeshik@snu.ac.kr
|
| Organization name |
Seoul National University
|
| Department |
School of Biological Sciences
|
| Lab |
Hyeshik Chang Lab
|
| Street address |
Building 203 Room 525, School of Biological Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu
|
| City |
Seoul |
| State/province |
South Korea |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
| |
|
| Platforms (1) |
| GPL8786 |
[miRNA-1] Affymetrix Multispecies miRNA-1 Array |
|
| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE35533 |
Analysis of small RNA through the cell cycle in HeLa |
|
| Relations |
| BioProject |
PRJNA156089 |
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