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Series GSE3554 Query DataSets for GSE3554
Status Public on Nov 04, 2005
Title Microarray Analysis of Retinal Gene Expression in the DBA/2J Model of Glaucoma
Organism Mus musculus
Experiment type Expression profiling by array
Summary Purpose: The DBA/2J mouse is a model for secondary angle-closure glaucoma due to iris atrophy and pigment dispersion, which ultimately leads to increased intraocular pressure (IOP). We sought to correlate changes in retinal gene expression with glaucoma-like pathology by performing microarray analysis of retinal RNA from DBA/2J mice at 3 months before disease onset, and at 8 months, after IOP elevation. Methods: IOP was monitored monthly in DBA/2J animals by Tono-Pen and animals with normal (3 months) or elevated IOP (8 months) were identified. RNA was prepared from 3 individual retinas at each age, and the RNA was amplified and used to generate biotin-labeled probe for high density mouse Affymetrix arrays (U430.2). A subset of genes was selected for confirmation by quantitative RT-PCR using independent retina samples from DBA/2J animals at 3, 5 and 8 months of age, and compared to retinas from C57BL/6J control animals at 3 and 8 months. Results: There were changes in expression of 68 genes, with 32 genes increasing and 36 genes decreasing at 8 months versus 3 months. Upregulated genes were associated with immune response, glial activation, signaling and gene expression, while down-regulated genes included multiple crystallin genes. Significant changes in 9 upregulated genes and 2 downregulated genes were confirmed by quantitative RT-PCR, with some showing changes in expression by 5 months. Conclusions: DBA/2J retina shows evidence for glial activation and an immune-related response following IOP elevation, similar to what has been reported following acute elevation of IOP in other models.
Keywords: retina, glaucoma, DBA/2J, elevated intraocular pressure
 
Overall design IOP was monitored monthly in DBA/2J animals by Tono-Pen and animals with normal (3 months) or elevated IOP (8 months) were identified. RNA was prepared from 3 individual retinas at each age, and the RNA was amplified and used to generate biotin-labeled probe for high density mouse Affymetrix arrays (U430.2). A subset of genes was selected for confirmation by quantitative RT-PCR using independent retina samples from DBA/2J animals at 3, 5 and 8 months of age, and compared to retinas from C57BL/6J control animals at 3 and 8 months.
 
Contributor(s) Steele MR, Inman DM, Calkins DJ, Horner PJ, Vetter ML
Citation(s) 16505032
Submission date Nov 03, 2005
Last update date Feb 11, 2019
Contact name Monica L. Vetter
E-mail(s) monica@neuro.utah.edu
Phone (801) 581-4984
Organization name University of Utah
Department Neurobiology & Anatomy
Lab Vetter Lab
Street address 20 South, 2030 East Rm. 320 BPRB
City Salt Lake City
State/province UT
ZIP/Postal code 84112-9458
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM81688 retina_DBA_543L_8mo_rep1
GSM81689 retina_DBA_544R_8mo_rep2
GSM81690 retina_DBA_552R_8mo_rep3
Relations
BioProject PRJNA93629

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE3554_RAW.tar 34.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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