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| Status |
Public on May 17, 2012 |
| Title |
Microbiota-dependent gene expression in small intestinal epithelial cells (IECs) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Intestinal epithelial cells were isolated from total small intestine of each four 28-day old conventionally raised (conv) and germ-free (GF) bred C57BL/6 mice (protocol according to: Lotz et al., J. Exp Med. 2006). Total RNA was isolated by TriZol and ist purity was examined using a Bioanalyszer. We used microarrays to comparatively detail the global gene expression in primary total isolated intestinal epithelial cells.
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| Overall design |
Four biological replicates from isolated intestinal epithelial cells obtained from each 4 germ-free bred and conventionally raised mice. Colour change.
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| Contributor(s) |
Hornef MW, Bäckhed F |
| Citation(s) |
22570612, 25210785 |
| Submission date |
Feb 07, 2012 |
| Last update date |
May 10, 2018 |
| Contact name |
Mathias W. Hornef |
| E-mail(s) |
hornef.mathias@mh-hannover.de
|
| Phone |
0049 511 538 9094
|
| Fax |
0049 511 532 4366
|
| Organization name |
Hannover Medical School
|
| Department |
Inst. of Medical Microbiology
|
| Street address |
Carl Neuberg Str. 1
|
| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA152515 |
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