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| Status |
Public on Feb 09, 2012 |
| Title |
HIGHLY DIVERSE TCRalpha CHAIN REPERTOIRE OF PRE-IMMUNE CD8+ T CELLS REVEALS NEW INSIGHTS IN GENE RECOMBINATION |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
By sequencing tens of millions of TCRα chain transcripts from naïve mouse CD8+ T cells, we observed a hugely diverse repertoire, comprising nearly all possible TRAV-TRAJ combinations. Our findings are not compatible with sequential coordinate gene recombination, but rather with a model in which contraction and DNA looping in the TCRαδ locus provide equal access to TRAV and TRAJ gene segments, similar to that demonstrated for IgH gene recombination
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| |
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| Overall design |
High-throughput sequencing of entire TCRa repertoire from C57Bl/6 mice
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| Contributor(s) |
Genolet R, Stevenson BJ, Farinelli L, Osteras M, Luescher IF |
| Citation(s) |
22373576 |
| Submission date |
Feb 08, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Brian James Stevenson |
| E-mail(s) |
brian.stevenson@unil.ch
|
| Organization name |
Swiss Institute of Bioinformatics
|
| Street address |
Genopode, UNIL-Sorge
|
| City |
Lausanne |
| ZIP/Postal code |
CH-1015 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| SRA |
SRP010815 |
| BioProject |
PRJNA152469 |
