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| Status |
Public on Feb 04, 2006 |
| Title |
Enigma (CG9006) RNAi vs control RNAi in Drosophila Kc-167 cells. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
5 day RNAi treatment to knockdown Enigma, CG9006, a Drosophila mitochondrial protein with homology to acyl-CoA dehydrogenases.
Keywords: Genetic modification
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| |
|
| Overall design |
Kc-167 cells, 5 day treatment with Enigma-specific RNAi or beta-lactamase control RNAi. Specifics:1.6X10^6 cells in 0.5mL of serum-free Sang's M3 media incubated for 1 hour with 20ug of dsRNA, subsequently equal volume of M3 media containing 10% serum was added. Cells harvested after 5 days. RNAi efficiency measured by immunoblot for Enigma.
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| |
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| Contributor(s) |
Mourikis P, Hurlbut GD, Artavanis-Tsakonas S |
| Citation(s) |
16434470 |
| Submission date |
Nov 04, 2005 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Spyros Artavanis-Tsakonas |
| E-mail(s) |
tsakonas@helix.mgh.harvard.edu
|
| Organization name |
Massachusetts General Hospital
|
| Department |
MGH Cancer Research Center
|
| Lab |
Artavanis-Tsakonas
|
| Street address |
7308 Building 149, 13th Street
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL72 |
[DrosGenome1] Affymetrix Drosophila Genome Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA93639 |
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