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Series GSE35669 Query DataSets for GSE35669
Status Public on Feb 10, 2012
Title Global comparative transcriptome analysis of cartilage formation in vivo
Organism Mus musculus
Experiment type Expression profiling by array
Summary We set out to characterize the gene expression changes which take place during chondrogenesis in the developing mouse limb. RNA derived from pre-condensed mesenchyme, mesenchymal condensations, and cartilage anlagen representing the earliest stages of tibial and fibular development was analysed by whole genome microarray analysis, and revealed 931 genes differentially expressed in these tissues. Among them were 892 genes not previously identified during the initation of chondrogenesis, including members of the Bmp, Wnt, Gdf, Sox, and Fox gene families. These microarray data were validated by qPCR, in situ hybridisation, and analysis of numerous genes already implicated in chondrogenesis in the scientific literature.
Overall design 231 sections from a total of four 11.5dpc mouse hind limbs, three 12.5dpc mouse hind limbs, and four 13.5dpc mouse hind limbs (all from separate mice) were microdissected, and tissues from each time point were pooled. Thus, this experiment consisted of one replicate only.
Contributor(s) Cameron TL, Belluoccio D, Farlie PG, Brachvogel B, Bateman JF
Citation(s) 19272164
Submission date Feb 09, 2012
Last update date May 10, 2018
Contact name Trevor L Cameron
Organization name Murdoch Childrens Research Institute
Street address Flemington Road
City Parkville
ZIP/Postal code 3052
Country Australia
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (6)
GSM873264 11.5dpc PCM vs 12.5dpc MC (Dye Combination 1)
GSM873265 11.5dpc PCM vs 13.5dpc CA (Dye Combination 1)
GSM873266 12.5dpc MC vs 11.5dpc PCM (Dye Combination 2)
BioProject PRJNA152441

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35669_RAW.tar 43.0 Mb (http)(custom) TAR (of GPR)
Raw data provided as supplementary file
Raw data included within Sample table
Processed data not provided for this record

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