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| Status |
Public on Jul 01, 2013 |
| Title |
Suppression of the antiviral response by an influenza "histone mimic" |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Viral infection is commonly associated with virus-driven hijacking of host proteins. We describe a novel mechanism by which influenza virus impacts host cells through the interaction of influenza NS1 protein with the infected cell epigenome. We show that the NS1 protein of influenza A H3N2 target the transcription elongation PAF1 complex (hPAF1C). We demonstrate that binding of NS1 to hPAF1C results in suppression of hPAF1C-mediated transcriptional elongation. In the following data sets, we show that NS1 colocalizes with hPAF1 on the chromatin of infected cells and that siRNA-mediated reduction of hPAF1 expression results in reduced recruitment of NS1 to the chromatin.
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| Overall design |
Examination of different histone modifications in infected cells and RNA-Seq and GRO-Seq transcript measurements.
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| Contributor(s) |
Marazzi I, Ho J, Kim J, Manicassamy B, Dewell S, Albrecht RA, Seibert C, Schaefer U, Jeffrey KL, Prinjha RK, Lee K, García-Sastre A, Roeder R, Tarakhovsky A |
| Citation(s) |
22419161 |
| Submission date |
Feb 13, 2012 |
| Last update date |
Jul 29, 2020 |
| Contact name |
ivan marazzi |
| E-mail(s) |
imarazzi@rockefeller.edu
|
| Phone |
2123278265
|
| Fax |
2123278258
|
| Organization name |
rockefeller univeristy
|
| Lab |
epigenetic and immune signaling
|
| Street address |
1230 York Avenue
|
| City |
new york |
| State/province |
ny |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (17)
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| This SubSeries is part of SuperSeries: |
| GSE35268 |
Analysis of global gene expression profiles of hPAF1 deficient A549 cells |
|
| Relations |
| SRA |
SRP010907 |
| BioProject |
PRJNA155897 |
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