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| Status |
Public on Jan 03, 2013 |
| Title |
Expression data from Bovine leukemia virus (BLV) Tax-transfected HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Bovine leukemia virus (BLV) Tax is a transcriptional activator of viral replication and a key contributor to oncogenic potential. We previously identified interesting mutant forms of Tax with elevated (TaxD247G) or reduced (TaxS240P) transactivation effects on BLV replication and propagation. In this study, to identify genes that play a role in the cascade of signal events regulated by wild-type and mutant Tax proteins, we used a large-scale host cell gene-profiling approach.
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| Overall design |
HeLa cells transiently transfected with the pCAGGS vector for the control sample was compared with HeLa cells transiently transfected with the pCAGGS-Tax-FLAG vector. These cells were incubated for 30h after transfection and total RNA was isolated.
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| Contributor(s) |
Aida Y, Arainga-Ramirez M, Fukumoto K |
| Citation(s) |
22455445 |
| Submission date |
Feb 14, 2012 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Yoko Aida |
| E-mail(s) |
aida@riken.jp
|
| Phone |
+81-48-462-4408
|
| Fax |
+81-48-462-4399
|
| Organization name |
RIKEN
|
| Department |
Advanced Science Institute
|
| Lab |
Viral Infectious Diseases Unit
|
| Street address |
2-1 Hirosawa
|
| City |
Wako |
| State/province |
Saitama |
| ZIP/Postal code |
351-0198 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (4)
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| GSM875738 |
pCAGGS-IRES-CFP transfected HeLa cells |
| GSM875739 |
pCAGGS-wild type BLV Tax-IRES-CFP transfected HeLa cells |
| GSM875740 |
pCAGGS-BLV high transactivation activity Tax(D247G) mutant-IRES-CFP transfected HeLa cells |
| GSM875741 |
pCAGGS-BLV low transactivation activity Tax(S240P) mutant-IRES-CFP transfected HeLa cells |
|
| Relations |
| BioProject |
PRJNA152159 |
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