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| Status |
Public on Feb 16, 2012 |
| Title |
Epigenetic silencing of PKD1 in autosomal dominant polycystic kidney disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
ADPKD (Autosomal dominant polycystic kidney disease) is the most common inherited disorders and is characterized by growth of numerous cysts filled with fluid in the kidneys. Ultimately, it leads to kidney failure. The mutations of PKD1 and PKD2 account for approximately 85 and 15 percent of ADPKD, respectively. However, the mechanisms related to genetic mutation of PKD1 and PKD2 are still unclear.
To investigate altered gene expression levels, Affymetrix microarray was performed using the kidney tissue from normal and ADPKD patients.
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| Overall design |
Total RNAs were isolated from kidney of human normal (49 years old/ male) and ADPKD patients (62 years old/ male, 67 years old/ male) using NucleoSpin® RNA Kit (MACHEREY-NAGEL) according to the manufacturer’s instructions. We used tha Affymetrix GeneChip Human Gene 1.0 ST Array. Per RNA sample, 300 ng was used as the input into the Affymetrix procedure as recommended by the manufacturer's protocol.
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| Contributor(s) |
Woo YM, Park JH |
| Citation missing |
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| Submission date |
Feb 15, 2012 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Yu Mi Woo |
| E-mail(s) |
milk-mi818@hanmail.net
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| Phone |
+82-2-710-9414
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| Fax |
+82-2-2077-7258
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| Organization name |
Sookmyung Women's University
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| Street address |
Cheongpa-ro 47-gil 100 (Cheongpa-dong 2(i)-ga), Yongsan-gu
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| City |
Seoul |
| ZIP/Postal code |
140-742 |
| Country |
South Korea |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA151897 |
